广东医学2018，Vol.39Issue(4)：501-505,5.
白藜芦醇对碘普罗胺诱导人肾小管上皮HK-2细胞氧化应激及Klotho蛋白表达的影响
The effect of resveratrol on oxidative stress and Klotho expression in iopromide-induced human renal tubular epithelial HK-2 cells
摘要
Abstract
Objective To investigate the effect of resveratrol on oxidative stress and Klotho expression in iopromide-induced human renal tubular epithelial HK-2 cells. Methods Different doses of iopromide ( 137. 5 mgI /mg, 275. 0 mgI /mg and 550. 0 mgI /mg) were added in human renal tubular epithelial HK-2 cells for 12 h to mimic contrast-induced nephropathy in vitro. MTT assay was used to measure the cell viability. Kit assay was used to measure lactate dehydrogenase enzyme ( LDH) vitality and flow cytometry was used to measure percentage of apoptosis. Different doses( 1 μmol /L, 10 μmol /L and 100 μmol /L) of resveratrol or N-acetylcysteine ( NAC) was added, and the cell viability, LDH vitality and percentage of apoptosis were detected. CM-H2DCFDA assay was used to measure cell fluorescence intensity of reactive oxygen species ( ROS) . Western bolt assay was used to measure the expression of Klotho protein. Results Iopromide treatment for 12 h in human renal tubular epithelial HK-2 cells caused cell viability decrease, and elevated LDH vitality and apoptosis in a dose-dependent manner. When different doses of resveratrol was added, the above cell changes were inhibited in a dose-dependent manner. The effect of resveratrol was similar to the NAC. In addition, iopromide treatment caused ROS fluorescence intensity upregulation, which was inhibited by both resveratrol and NAC. The Klotho protein expression was reduced in the iopromide group when compared with the control group, which was inhibited by resveratrol but not NAC. Conclusion Resveratrol alleviates iopromide-induced toxic effects on HK-2 cells as the same as NAC. It may be related to its effects on upregulation of Klotho protein and downregulation of oxidative stress in HK-2 cells.关键词
白藜芦醇/碘普罗胺/人肾小管上皮HK-2细胞/Klotho蛋白/氧化应激/凋亡Key words
resveratrol/iopromide/human renal tubular epithelial HK-2 cells/Klotho/oxidative stress/apoptosis引用本文复制引用
张滨,钟小华,廖丽贞..白藜芦醇对碘普罗胺诱导人肾小管上皮HK-2细胞氧化应激及Klotho蛋白表达的影响[J].广东医学,2018,39(4):501-505,5.基金项目
广东省中医药局建设中医药强省立项资助科研课题(编号:20152155) （编号:20152155）
