广东医学2018，Vol.39Issue(4)：511-515,5.
耐药结核分枝杆菌多重Taqman-MGB探针检测方法的建立
Establishment of multiple Taqman-MGB probe detection method for drug resistant Mycobacterium tuberculosis
摘要
Abstract
Objective To establish a multiple Taqman-MGB probe detection system for drug resistant Mycobacterium tuberculosis. Methods The primers and probes targeting mutation sites of Mycobacterium tuberculosis against tuberculosis first-line drug[rifampicin: RpoB ( 526) /( 531); ethambutol: embB ( 306); isoniazid: KatG ( 315) , inhA(-15); streptomycin: RpsL ( 43)]were designed and synthesized. Wild-type and mutant templates of drug resistant Mycobacterium tuberculosis were constructed and diluted. Drug resistance gene detection reagent of Mycobacterium tuberculosis was prepared, and tested by different templates. 72 tuberculosis samples ( rifampin resistance 20 strains; streptomycin resistance 22 strains; isoniazid resistance 1 strain; multiple drug resistance 17 strains) were analyzed by the reagent. The results were compared with Roche efficacy test. Results The detection system could simultaneously detect 6 tuberculosis mutation sites, and the minimum detection limit was 104 copies /mL. In clinical samples, the total compliance rate was 100％ with good consistency (χ2 =137. 3, P < 0. 001) . Conclusion This study establishes a rapid detection method of drug resistant Mycobacterium tuberculosis, which can be used for health quarantine system and clinical detection.关键词
结核分枝杆菌/耐药结核分枝杆菌/Taqman-MGB/快速检测Key words
Mycobacterium tuberculosis/drug resistant Mycobacterium tuberculosis/taqman-MGB/quick detection引用本文复制引用
孙菲,易松林,李丹,戴佩希,彭飞武,唐连飞..耐药结核分枝杆菌多重Taqman-MGB探针检测方法的建立[J].广东医学,2018,39(4):511-515,5.基金项目
国家质量监督检验检疫总局科技计划项目(编号:2015IK125),湖南省科技重点研发计划项目(编号:2015SK2055) （编号:2015IK125）
