广东医学2018，Vol.39Issue(4)：524-527,4.
NLRP3在膀胱癌中的表达及意义
The clinical significance and expression of NLRP3 in bladder cancer
摘要
Abstract
Objective To study the expression of NLRP3 in bladder cancer cell lines and its effects on proliferation, migration and invasion of bladder cancer cells. Methods 31 cases of bladder cancer tissue samples and 8 adjacent tissues were obtained from the patients who underwent surgical resection. The real-time fluorescence quantitative PCR( qPCR) and Western blot were used to assess the expression of NLRP3. The correlation between the clinical data and the expression of NLRP3 was analyzed. In vitro, bladder cancer cells ( T24, 5637 and BIU-87) were used to investigate the effects of NLRP3 on proliferation, migration and invasion of cancer cells. Results Compared with the adjacent tissues, the expression of NLRP3 mRNA and protein in bladder cancer tissues was significantly up-regulated. Statistical analysis showed that the expression of NLRP3 mRNA was significantly correlated with pathological stage ( pT) , lymph node status( pN) and WHO tumor grading ( all P < 0. 01) . The expression of NLRP3 in tumor cell lines was significantly higher than that in normal bladder epithelial cells ( P < 0. 01) . The expression of NLRP3 in 5637 and T24 cells was increased significantly( P < 0. 01) . Silencing of NLRP3 significantly inhibited cell proliferation, migration and invasion of 5637 and T24 cells in vitro. Conclusion The expression of NLRP3 is closely correlated to the malignant characteristics of bladder cancer, the proliferation, migration and invasion ability of bladder cancer, and it is expected to be a potential target for bladder cancer treatment.关键词
膀胱癌/NLRP3/细胞系/增殖/迁移和侵袭Key words
bladder cancer/NLRP3/cell line/proliferation/migration and invasion引用本文复制引用
张惟,贾招辉,张建国,高中伟..NLRP3在膀胱癌中的表达及意义[J].广东医学,2018,39(4):524-527,4.基金项目
河南省自然科学基金面上项目(编号:162300410106) （编号:162300410106）
