广东医学2018，Vol.39Issue(10)：1441-1444,4.
大鼠妊娠高血压胎盘脂代谢与脂肪酸结合蛋白4的相关性分析
The correlation analysis between abnormal lipid metabolism and FABP4 expression in placenta tissue of rats with hypertension of pregnancy
摘要
Abstract
Objective To investigate the correlation between placenta tissue lipid metabolic disorder and lard type fatty acid binding protein 4 (FABP4) and its role in hypertension of pregnancy .Methods Twenty female SD rats (weight range from 250-300 g, SPF grade) were randomly divided into 2 groups (n＝10 for each group), control group (Group PC) and hypertension of pregnancy group (Group HDCP).The rats in the 2 groups were injected with normal sa-line and nitric oxide synthase inhibitor (L-NAME) 50 mg/( kg· d) to induced hypertension of pregnancy model (HD-CP), respectively.Blood pressure and urine protein were recorded .Placental tissues were collected for HE staining .En-zymatic method was used to assess the total cholesterol (TC), free cholesterol (FC), cholesterol ester (CE), free fatty acid (FFA) and triglyceride (TG) content in placental tissues .qRT-PCR and Western blot were used to detected the expression of FABP4 in placental tissue.The correlation between the expression of FABP 4 protein and the contents of TC and TG in placenta tissues were analyzed .Results Compared with Group PC, the blood pressure and urine protein of Group HDCP were significantly elevated (P＜0.05).HE staining showed that the decrease in the number of placental vil-li and unclear structure in Group HDCP .The contents of TC, FC, CE, FFA and TG and the expression of FABP4 were al-so significantly elevated in Group HDCP compared with Group PC (P＜0.05).There were significantly positive correla-tions between TC or TG and FABP4 protein level.Conclusion Abnormal lipid metabolism may play an important role in the pathogenesis of HDCP .FABP4 may affect the lipid metabolism , which leads to the occurrence and development of HDCP.关键词
妊娠高血压/SD大鼠/胎盘组织/脂代谢异常/脂肪型脂肪酸结合蛋白4Key words
hypertension of pregnancy/SD rat/placenta tissue/abnormal lipid metabolism/fatty acid binding protein 4引用本文复制引用
高婷婷,徐华,张慧萍,宋艳春,王艳华,张辉,惠淑宁,马燕琼,马晓莉,吴凯,张鸣号..大鼠妊娠高血压胎盘脂代谢与脂肪酸结合蛋白4的相关性分析[J].广东医学,2018,39(10):1441-1444,4.基金项目
国家自然科学基金资助项目(编号:81660258),优势学科群建设科研项目(编号:XY201506,XY201415) （编号:81660258）
