干旱地区农业研究2018，Vol.36Issue(3)：59-65,7.DOI:10.7606/j.issn.1000-7601.2018.03.09
玉米MYB转录因子靶基因的全基因组预测及验证
Genome-wide prediction and verification for target genes of MYB transcription factor in maize
摘要
Abstract
The MYB transcription factor are involved in the regulation of plant growth,and stress response.To predict the MYB target genes and their promoters within the whole genome of maize.,the HexDIFF algorithm was combined with support vector machines to construct a classification model based on the verified core sequences of MYB recognition sites and their flanking sequences.The results showed that a total of 435 MYB binding sites were obtained and 424 genes downstream of them were identified as MYB target genes,involving in various growth and development processes.To verify the prediction results,the electrophoretic mobility shift assay was performed to de-tect the combination between maize MYB-IF25 protein and 30 predicted binding sites.As a result,27 binding sites could interact with MYB-IF25 protein.GUS transient expression showed that the promoters of predicted MYB stress-related target genes could initiate the GUS gene expression,indicating that these promoters possessed driving activity.The above results prove that the predicion method has high reliablity and can provide a reference for pre-dicting the target gene of transcription factor.关键词
基因组/玉米/MYB转录因子/支持向量机/靶基因Key words
genome/maize/MYB transcription factor/support vector machine/target gene分类
农业科技引用本文复制引用
张晓芳,于好强,罗羲,张于福,李晚忱,付凤玲..玉米MYB转录因子靶基因的全基因组预测及验证[J].干旱地区农业研究,2018,36(3):59-65,7.基金项目
国家科技重大专项(2016ZX08003-004) （2016ZX08003-004）
国家自然科学基金(31071433) （31071433）
