干旱地区农业研究2018，Vol.36Issue(3)：130-138,9.DOI:10.7606/j.issn.1000-7601.2018.03.20
黄瓜枯萎病拮抗菌的拮抗促生因子检测及生物效应研究
Determining antagonistic and growth promoting factors of bacteria strains against cucumber Fusarium wilt and studies on their biological effects
摘要
Abstract
The strains with antagonistic activity against cucumber Fusarium wilt pathogen(FOC,Fusarium oxys-porum f.sp.cucumerinum)were screened.Two strains named QHZ-Yb1 and QHZ-Yb2 were obtained by primary and repeated screening,which showed stable and strong antagonism.It produced inhibition zones with respective width of 3.37mm and 3.42mm and their diameter more than 2.7cm,and the inhibition rates were up to 61.9％and 62.3％by the plate confrontation method.Two strains were preliminarily identified as Bacillus atrophaeus based on the morphological,physiological and biochemical identifications,and the 16S rDNA sequence analyse.In addition,the experiment determining antagonistic and growth promoting factors was conducted to examine the capacity of the strains.The results showed that Yb1 and Yb2 produced multiple antagonistic and growth promoting factors.The anti-bacterial rate of lipopeptide extracted from the two strains were 57.4％and 58％respectively.The secretion of growth hormone(IAA)were 1.56mg·L-1and 3.69mg·L-1respectively.The amount of solubilizing P were 0.77mg·L-1 and1.19mg·L-1respectively.Siderophore and casease can also be produced in two strains.Seed germination test showed that the bacteria suspension of Yb1 and Yb2 could promote the germination of cucumber seeds.According to the pot experiment of bacteria's biological effect,Whether the strains were combined or not,it not only had obvi-ously biocontrol effect on cucumber Fusarium wilt,but also significantly increased plant biomass and the amount of bacteria and actinomycetes in cucumber plants rhizosphere,reduced the number of fungi and Fusarium oxysporum.The biocontrol efficiency using the combination of two strains was significantly higher than that of Yb1 or Yb2,which made the incidence of cucumber fusarium wilt reduce 11.11％and the plants biomass increase 56.7％.In conclusion,both Yb1 and Yb2 have well biocontrol potential against FOC.Moreover,Yb1 and Yb2 in combination showed the highest efficiency compared with Yb1 or Yb2 alone.关键词
尖孢镰刀菌黄瓜专化型/黄瓜枯萎病/拮抗菌/萎缩芽孢杆菌Key words
Fusarium oxysporum f.sp.cucumerinum/cucumber Fusarium wilt/antagonistic bacteria/Bacillus atrophaeus分类
农业科技引用本文复制引用
马兴,董星晨,张健,罗超越,邓德雷,张春红,王友玲,邱慧珍..黄瓜枯萎病拮抗菌的拮抗促生因子检测及生物效应研究[J].干旱地区农业研究,2018,36(3):130-138,9.基金项目
国家重点基础研究发展计划(973计划)(2015CB150501) （973计划）
国家自然科学基金(31360500) （31360500）
国家公益性行业(农业)科研专项(201103004) （农业）
白银市白银区科技计划项(2014-5N) （2014-5N）
甘肃农业大学校创新基金(GAU-CX1021) （GAU-CX1021）
