干旱地区农业研究2018，Vol.36Issue(3)：184-193,229,11.DOI:10.7606/j.issn.1000-7601.2018.03.28
不同外源调节物质对低温胁迫下玉米的缓解效应分析
Mitigation effect analysis of different exogenous regulatory substances on maize under low-temperature stress
摘要
Abstract
Proline(Pro)and Glycine betaine(GB)are important osmoregulation substances that are associ-ated with cold resistance in plants.To investigate the effect of exogenous Pro and GB on maize germination and seedling traits under low-temperature stress,and to comprehensively evaluate the cold mitigation effect of Pro and GB.Three maize chilling sensitive lines were used with five different concentrations of Pro or GB's soak or culture treatment under 10℃ stress at germination or seedling stage.Double distilled water soak and culture were used as positive and negative control check(CK(+)and CK(-))under 25℃ and 10℃ at two stages.It showed that:(1)The germination potential(GP),germination rate(GR),plumule length(PL),radical length(RL),shoot fresh weight(SFW),root fresh weight(RFW)of maize seed significantly decreased,and relative conductivity(RC),malondialdehyde content(MDA),proline content(Pro),soluble sugar content(SS),SOD,POD and CAT activ-ity of seedling significantly increased under low-temperature stress.(2)RC and MDA significantly decreased,and other five traits of seedling and six traits of seed germination significantly increased after adding exogenous Pro and GB under low-temperature stress.(3)The six germination traits and seven physiological and biochemical traits showed significant difference in inbred lines,in the concentrations of Pro and GB,and in the interaction of inbred lines and concentration of Pro and GB.(4)Cold ease index(EI)was used as comprehensive evaluation index,and membership function method was used to comprehensive evaluate the exogenous Pro and GB's mitigation effect to colde stress.600,800μmol·L-1of Pro and 20μmol·L-1of GB had the best cold mitigation effect of 0.882 and 0.647,and 1000μmol·L-1of Pro and 50μmol·L-1of GB had the worst cold mitigation effect of 0.513 and 0.022 at germination.400μmol·L-1of Pro and 10μmol·L-1of GB had the best cold mitigation effect of 0.577 and 0.500,and 1000μmol·L-1of Pro and 40μmol·L-1of GB had the worst cold mitigation effect of 0.246 or 0.289 at seedling stage.The cold mitigation effect of Pro was 1.858 and 1.064 times of GB at germination and seedling stage,respectively.关键词
玉米/低温胁迫,外源脯氨酸/甜菜碱/缓解指数/综合评价Key words
maize/low-temperature stress/exogenous Pro/Glycine betaine( GB)/mitigation index/compre-hensive evaluation分类
农业科技引用本文复制引用
赵小强,彭云玲,方鹏,武博洋,闫慧萍..不同外源调节物质对低温胁迫下玉米的缓解效应分析[J].干旱地区农业研究,2018,36(3):184-193,229,11.基金项目
国家自然基金项目(31260330,31301333) （31260330,31301333）
教育部博士点基金(20126202120001) （20126202120001）
甘肃省国际合作项目(1504WKCA009) （1504WKCA009）
甘肃省重大科技专项(17ZD2NA016) （17ZD2NA016）
伏羲"青年英才"项目(GAUFX-02Y09) （GAUFX-02Y09）
