广东药科大学学报2018，Vol.34Issue(3)：288-292,5.DOI:10.16809/j.cnki.2096-3653.2018030702
水溶性灵芝粉的制备及其免疫调节活性评价
Preparation of water-soluble Ganoderma lucidum powder and its immunomodulatory effect
摘要
Abstract
Objective To analyze the main components of self-made water-soluble G. lucidum powder and evaluate its immunomodulatory activity. Methods Ganoderma lucidum was used as the raw material, and the water-soluble G. lucidum powder was prepared through hot water extraction, high-speed centrifugation, rotary evaporation, and freeze-drying. The crude polysaccharide, reducing sugar and total flavonoids of G. lucidum powder were determined by national standard method, and the method of the health food inspection and evaluation technical specifications ( 2003 version) was used to evaluate the immune regulatory activity of G. lucidum powder. Results The results of physical and chemical analysis showed that the contents of crude polysaccharide, triterpenoids, total flavonoids, reducing sugars, and soluble protein of the water-soluble G. lucidum powder were 36. 6, 24. 8, 17. 7, 9. 2, 2. 4 g/100 g, respectively. The results of animal experiments showed that compared with the control group, medium ( 10 mg/kg) and high dose ( 20 mg/kg) significantly increased the phagocytic capacity of mice, killing activity of NK cells, spleen lymphocyte transformation, serum hemolysin content, and mouse paw thickness, the difference was statistically significant ( P<0.01) . Compared with the control group, the organ index was improved, but the difference was not statistically significant. Conclusion The water-soluble G. lucidum powder polysaccharide and the Ganoderma lucidum triterpene have higher quality, and it has good immunoregulatory activity.关键词
水溶性灵芝粉/灵芝多糖/灵芝三萜/免疫调节Key words
water-soluble Ganoderma lucidum powder/ganoderma polysaccharide/ganoderma triterpene/immune activity分类
医药卫生引用本文复制引用
何嘉烽,吴小勇,尹辉,李文治,何文江..水溶性灵芝粉的制备及其免疫调节活性评价[J].广东药科大学学报,2018,34(3):288-292,5.基金项目
国家自然科学基金面上项目(81770527) （81770527）
