广东药科大学学报2018，Vol.34Issue(3)：303-306,4.DOI:10.16809/j.cnki.2096-3653.2018031901
UHPLC-MS-MS法同时测定舒筋活血颗粒中葛根素和黄芪甲苷的含量
Simultaneous determination of puerarin and astragaloside A in Shujin Huoxue Keli by UHPLC-MS-MS spectrometry
摘要
Abstract
Objective To establish quality evaluation method for Shujin Huoxue Keli. Methods UHPLC-MS-MS was used for simultaneous determination of puerarin and astragaloside A, two active components of Shujin Huoxue Keli. An Agilent poroshell C18column ( 2.1 mm×100 mm, 2.7 μm) was used with a mobile phase containing 0.2% formic acid and acetonitrile ( 40 :60) , the separation was carried out in isocratic mode within 4 min, and 10 batches of Keli were analyzed. Results Puerarin and astragaloside A were eluted within 4 min, with good linearity ( 0.999 2 and 0.999 4, respectively) in the concentration range of 10-1 000 ng/mL. For 10 batches of Keli, the mean content of puerarin was 3.42 mg/g ( in dried granules) , RSD was 8.12%;the mean content of astragaloside A was 0.028 mg/g, RSD was 19.63%. Conclusions The study proposed an accurate, fast and effective method for simultaneous determination of main active components in Shujin Huoxue Keli, which provides a reference for the evaluation of complex TMC formula.关键词
舒筋活血颗粒/UHPLC-MS-MS法/葛根素/黄芪甲苷Key words
Shujin Huoxue Keli/UHPLC-MS-MS/puerarin/astragaloside A分类
医药卫生引用本文复制引用
陈昭,陈伟韬,胥爱丽,李养学,毕晓黎..UHPLC-MS-MS法同时测定舒筋活血颗粒中葛根素和黄芪甲苷的含量[J].广东药科大学学报,2018,34(3):303-306,4.基金项目
广东省科技计划项目:中药配方颗粒国家标准研究与制定( 2015B070701010) （ 2015B070701010）
佛山市科技创新专项资金(2013GQ100032) （2013GQ100032）
广东省科技计划项目:创新中药研发平台建设(2017A070701017) （2017A070701017）
广东省科技计划项目:广东省医疗机构中药制剂研发技术平台建设(2013B040200040) （2013B040200040）
