广东药科大学学报2018，Vol.34Issue(3)：316-319,4.DOI:10.16809/j.cnki.2096-3653.2018030603
白头翁皂苷D体外抗肺腺癌作用及其机制
Antitumor effect and mechanism of pulchinenoside D on A549 tumor cells in vitro
摘要
Abstract
Objective To study the antitumor effect and mechanism of pulchinenoside D on human lung tumor A549 cells in vitro. Methods MTT assay was used to evaluate the increment restrain of pulchinenoside D on A549 cells. Colony formation units were used to examine colony-forming role by pulchinenoside D on A549 cells. Flow cytometry assay was used to analyze the apoptosis rate of A549 cells. Western blotting was employed to determine the expression levels of bcl-2, caspase-3 and the proteins related to the PI3K/Akt/mTOR signal in A549 cells. Results Pulchinenoside D restrained the proliferation of A549 cells, and significantly inhibited the colony formation of A549 cells. Pulchinenoside D at 20 and 40 μmol/L significantly induced the apoptosis of A549 cells. Pulchinenoside D at 20 and 40 μmol/L reduced the expression of bcl-2 and caspase 3. Conclusion Pulchinenoside D displays antitumor effects on A549 tumor cells in vitro. The mechanism of which is related to regulation of the mitochondrial apoptosis pathway and PI3K/Akt/mTOR signal.关键词
白头翁皂苷D/肺腺癌/凋亡/细胞周期/PI3K-Akt-mTOR途径Key words
pulchinenoside D/lung cancer/apoptosis/cell cycle/PI3K/Akt/mTOR signal分类
医药卫生引用本文复制引用
王彦儿,岳文华,许琼明,李笑然,李坤平..白头翁皂苷D体外抗肺腺癌作用及其机制[J].广东药科大学学报,2018,34(3):316-319,4.基金项目
国家自然科学基金项目(81273402,81573548) （81273402,81573548）
