临床与病理杂志2018，Vol.38Issue(1)：23-27,5.DOI:10.3978/j.issn.2095-6959.2018.01.005
定量检测膀胱尿路上皮癌患者手术前后外周血中survivin mRNA的临床意义
Clinical significance of survivin mRNA detection in the peripheral whole blood of patients with bladder urothelial carcinoma before and after surgery
摘要
Abstract
Objective: To investigate the mRNA expression of survivin in whole blood of patients with bladder urothelial carcinoma. Methods: Levels of survivin mRNA expression in the whole blood of 47 patients with non-muscleinvasive bladder cancer before and after operations and 25 normal controls were detected by qRT-PCR and the receiver operating characteristic (ROC) curve for each factor was constructed. Results: The levels of survivin mRNA in bladder cancer patients were 4.06 folds compared to normal controls (P＜0.05). The cutoff value for differentiating bladder cancer patients and normal people was 1.4xl0-2 for survivin. Postoperative survivin expression in patients was significantly lower than preoperative expression at each time point (P＜0.05). Post-operative recurrence rate of post-operative patients with mRNA level above cutoff value were higher than lower ones (P＜0.05). Conclusion: qRT-PCR is a convenient and sensitive method to detect survivin mRNA in the whole blood using, and survivin could be useful tumor biomarkers for early detection and recurrence of bladder cancer.关键词
膀胱癌/survivin/外周血/mRNA/qRT-PCRKey words
bladder urothelial carcinoma/survivin/peripheral blood/mRNA/qRT-PCR引用本文复制引用
陈平锋..定量检测膀胱尿路上皮癌患者手术前后外周血中survivin mRNA的临床意义[J].临床与病理杂志,2018,38(1):23-27,5.基金项目
衡阳市科技计划项目(2102KJ50).This work was supported by the Science and Technology Project Foundation of Hengyang, China (2102KJ50). （2102KJ50）
