临床与病理杂志2018，Vol.38Issue(2)：239-245,7.DOI:10.3978/j.issn.2095-6959.2018.02.003
微小RNA-504对非小细胞肺癌细胞增殖及凋亡的作用
Effect of microRNA-504 on proliferation and apoptosis of non-small cell lung cancer cells
摘要
Abstract
Objective: To investigate the effect and possible mechanism of microRNA-504 (miR-504) in proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells. Methods: Expression of miR-504 in three NSCLC cell lines (A549, H1299 and HCC827) and control human bronchial epithelial cell line BEAS-2B were determined by real-time PCR. The cell line (HCC827) with the most significant change of miR-504 expression was used in the following study. Then HCC827 cells were transfected with miR-504 mimic, miR-504 inhibitor and nontargeting miR-control by using Lipofectamine RNAiMAX. Cell proliferation of the three above-mentioned groups was detected by CCK-8 kit. Cell apoptosis of the three groups was measured by Annexin V-FITC/PI apoptosis detection kit. Expression of P53 of the three groups was determined by Western blot. Results: Compared with BEAS-2B control cells, expression of miR-504 was significantly increased in all three NSCLC cell lines. HCC827 cells were used in the following study due to its most significant increase of miR-504. Compared with miR-control group, over-expression of miR-504 (miR-504 mimic) resulted in accelerated proliferation and decreased apoptosis of HCC827 cells. While down-regulation of miR-504 (miR-504 inhibitor) led to retarded proliferation and increased apoptosis. In addition, expression of P53 was decreased in miR-504 mimic group but increased in miR-504 inhibitor group. Conclusion: miR-504 could promote proliferation and inhibit apoptosis of NSCLC cells, which was possibly mediated by down-regulating P53 expression.关键词
微小RNA-504/非小细胞肺癌/增殖/凋亡Key words
microRNA-504/non-small cell lung cancer/proliferation/apoptosis引用本文复制引用
高昕,杨智,于海波,才陆贤,王知勇,刘洪汐,王利斌,刘博..微小RNA-504对非小细胞肺癌细胞增殖及凋亡的作用[J].临床与病理杂志,2018,38(2):239-245,7.基金项目
辽宁省科学技术计划项目(2012225019).This work was supported by the Science and Technology Project of Liaoning Province, China (2012225019). （2012225019）
