广西科学2018，Vol.25Issue(3)：233-241,9.DOI:10.13656/j.cnki.gxkx.20180528.001
枯草芽孢杆菌表达系统及其启动子的研究进展
Research Progress of Bacillus subtilis Expression Sys-tem and Its Promoter Regulatory Elements
摘要
Abstract
Bacillus subtilis is an important model strain in industrial application.It has the char-acteristics of non-pathogenicity,clear genetic background,strong protein secretion ability,easy isolation and culture.It is an ideal host for heterologous protein expression and secretion.Effi-cient and controllable promoters are one of the key factors for the efficient expression of heter-ologous proteins.According to the induction mechanism,promoters can be divided into consti-tutive promoters,inducible promoters,auto-inducible promoters and stage specific promoters.This article briefly introduced the Bacillus subtilis expression system,Bacillus sigma factor type and Bacillus subtilis promoter structure,compared the advantages and disadvantages of commonly used promoters,summarized the methods for the cloning and transformation and bioinformatics prediction of new promoters,prospected the future research direction of the Bacillus subtilis promoter which laid the foundation for further research on the structure and function of promoters and industrial produc-tion of foreign aid proteins.关键词
枯草芽孢杆菌/表达系统/启动子Key words
Bacillus subtilis/expression sys-tem/promoter分类
生物科学引用本文复制引用
熊海涛,韦宇拓..枯草芽孢杆菌表达系统及其启动子的研究进展[J].广西科学,2018,25(3):233-241,9.基金项目
广西自治区主席科技资金项目(NO:16449-01)资助. （NO:16449-01）
