广西科学2018，Vol.25Issue(3)：242-247,257,7.DOI:10.13656/j.cnki.gxkx.20180522.001
基因重组型枯草芽孢杆菌芽孢表面展示技术与应用
Technique and Application of Recombinant Bacillus subtilis Spore Surface Display
摘要
Abstract
Bacillus subtilis is an aerobic,gram-positive probiotic,which can form spores on strong stress resistant when facing the nutritional starvation.Owing to the unique structure and physiological function of spores,spore surface display technologies have many advantages over other ones.The constructed recombinant spores are not only easy to purify,but also have high recovery rate and good safety.Bacillus subtilis surface display technique has been developed rapidly in recent years.In recent years,Bacillus subtilis spore surface display technology has been rapidly developed,and various anchored proteins such as CotB,CotC,CotG,CotZ,CotA,OxdD,CotE,CotZ,and CgeA have been successfully used to display foreign proteins or poly-peptides on spore surfaces and applied to the fields of industrial enzymes,oral vaccines and drugs,the production of large molecular weight polymer protein and biological treatment of environmental pollution and other fields.In this study,the integration strategy of the spore dis-play system is introduced firstly,and the applica-tions of anchored proteins in Bacillus subtilis spores display technology,as well as its applica-tion and prospect in various fields are expounded further.关键词
枯草芽孢杆菌/芽孢表面展示/基因重组/锚定蛋白应用Key words
Bacillus subtilis/spore surface dis-play/gene recombination/anchor protein applica-tion分类
生物科学引用本文复制引用
张国艳,齐向辉,张欢欢,员君华,杨苗苗,袁娇,郭明明,邹樊,周志成,李雯..基因重组型枯草芽孢杆菌芽孢表面展示技术与应用[J].广西科学,2018,25(3):242-247,257,7.基金项目
国家重点研发计划(2017YFC1600806),江苏省"六大人才高峰"项目(SWYY-018),国家自然科学基金项目(31571806)和中国博士后基金项目(2017M621657)资助. （2017YFC1600806）
