广西科学2018，Vol.25Issue(3)：299-303,5.DOI:10.13656/j.cnki.gxkx.20180612.002
重组脂肪酶自诱导发酵培养基优化
Optimization of Auto-induction Fermentation Medium for Recombined Lipase
摘要
Abstract
[Objective]Optimizing the auto-induction of the fermentation medium to increase li-pase enzyme production.[Methods]In this research,through the construction of a transposon-integrated lipase recombinant bacterial strain,using automatic induction fermentation expres-sion method combined with CCD response surface experimental design method,the carbon source in the auto-induction medium was optimized,and a quadratic model was obtained,which was used to describe the effect of the carbon source in the fermentation medium on lipase pro-duction.[Results]The optimized fermentation medium(W/V)contained glycerol 2.596,glu-cose 0.035,lactose 1.289,tryptone 1.0,yeast extract 0.5,Na2HPO4·12H2O 1.79,KH2PO4 0.68,NH4Cl 0.2675,Na2SO40.071,MgSO4·7H2O 0.05.[Conclusion]The results of the fermentation test confirmed that the lipase enzyme specific activity in the optimized fermen-tation medium was 6.35 U/mg,which was near-ly three times higher than that in the initial fer-mentation medium.关键词
脂肪酶/自动诱导/响应面/培养基优化Key words
lipase/auto-induction/response sur-face method/medium optimization分类
生物科学引用本文复制引用
潘勤春,刘兴胥,吕军,朱婧,王青艳,周礼芹..重组脂肪酶自诱导发酵培养基优化[J].广西科学,2018,25(3):299-303,5.基金项目
广西科学研究与技术开发计划项目(桂科合 15104001-18)资助. （桂科合 15104001-18）
