广西科学2018，Vol.25Issue(3)：318-324,7.DOI:10.13656/j.cnki.gxkx.20180528.002
TLR2和TLR6在鸟分枝杆菌诱导巨噬细胞凋亡中的作用
The Effect of TLR2 and TLR6 on Macobacterium avium Induced Macrophages Apoptosis
摘要
Abstract
[Objective]A Mycobacterium avium-infected macrophage model was established to investigate the role of Toll-like receptor 2(TLR2)and Toll-like receptor 6(TLR6)in the ap-optosis of macrophages induced by Mycobacterium avium and to provide a basis for clarifying the pathogenesis of Mycobacterium avium.[Methods]U937 cells were infected with Mycobacterium avium at 0 h,8 h,16 h,24 h,and 48 h time points,respectively,and then the total proteins of the cells were used to detect the ex-pression of TLR2 and TLR6 by Western blot.The cell culture supernatants were extracted re-spectively and the changes of tumor necrosis fac-tor(TNF-α)content were detected by ELISA.The cytokines TNF-αwas measured by ELISA from U937 cells supernatant.TLR2 and TLR6 monoclonal antibody were used to treat the U937 cells 1 h before Mycobacterium avium stimu-lation.The expression of BAX and BCL-2 was evaluated by Western blot.The cytokines of TNF-αwas measured by ELISA from U937 cells supernatant.U937 cell apoptosis rate was de-tected with flow cytometry.[Results]Mycobacterium avium infection of U937 cells could cause the upregulation of TLR2,TLR6,and TNF-α expression.Blocking U937 cells after TLR2 and TLR6 infection with Mycobacterium avium could cause the expression of proapoptotic factors BAX and TNF-αin U937 cells.The amount was decreased(P＜0.05),the expression level of anti-apoptosis factor BCL-2 was increased(P＜ 0.05),and the apoptosis rate of cells infected with Mycobacterium avium was significantly decreased(P ＜ 0.05).[Conclusion]TLR2 and TLR6 are associated with macrophage apoptosis,macrophages recognize M.avium by TLR2 and TLR6,whereas Mycobacterium avium in turn affects macrophage-associated apoptosis path-ways by promoting the expression of TLR2 and TLR6.关键词
鸟分枝杆菌/巨噬细胞/TLR2/TLR6/细胞凋亡Key words
Mycobacterium avium/macrophage/TLR2/TLR6/apoptosis分类
生物科学引用本文复制引用
宁雪萍,杨东君,张依,田爽,凌敏..TLR2和TLR6在鸟分枝杆菌诱导巨噬细胞凋亡中的作用[J].广西科学,2018,25(3):318-324,7.基金项目
国家自然科学基金项目(81260245)和广西自然科学基金项目(2015GXNSFAA139077)资助. （81260245）
