南方农业学报2018，Vol.49Issue(6)：1053-1060,8.DOI:10.3969/j.issn.2095-1191.2018.06.02
芒果乙烯受体ETR1和ERS1基因的克隆及序列分析
Cloning and sequence analysis of ethylene receptor ETR1 and ERS1 genes from mango(Mangnifera indica L.)
摘要
Abstract
[Objective]Ethylene receptor ETR1 and ERS1 genes(MiETR1 and MiERS1)from mango(Mangnifera in-dica L.)were cloned,and bioinformatics analysis was conducted in order to provide theoretical reference for further study of functional mechanism of the ethylene receptors in mango ripening and senescencing.[Method]Using mango fruit as material,full-length cDNA sequences of MiETR1 and MiERS1 by RACE technology was amplified,and the encoded ami-no acid sequences were predicted by bioinformatics method.[Result]The full-length cDNA sequence of cloned gene Mi-ETR1 was 2570 bp,an open reading frame(ORF)of 2220 bp,encoding 739 amino acids. The full-length cDNA sequence of cloned gene MiERS1 was 2171 bp,and it was consisted of an ORF of 1890 bp,encoding 629 amino acids. The Gen-Bank accession numbers for the two were KY002681 and KY002682. Bioinformatic analysis showed that the MiETR1 and MiERS1 proteins were non secreted hydrophobic protein with transmembrane domain,belonging to the ETR1 family member of ethylene receptor with Ser-based,Thr-assisted phosphorylation-modulatory. MiETR1 and MiERS1 proteins contained three typical modules,namely GAF domain,HisKA domain and HATPase_c domain. MiERS1 protein lacked REC domain compared with MiETR1. The amino acid sequences of ETR1 and ERS1 proteins in different species were highly conserved. MiETR1 and MiERS1 had high homologous with the ETR1 and ERS1 proteins of Citrus sinensis and Citrus clementina. However,the two clustered on two different branches,indicating that there was genetic differentiation between the two proteins at certain extents.[Conclusion]Ethylene receptor gene plays a regulatory role in mango fruit maturation and senescence.关键词
芒果/乙烯受体/基因克隆/序列分析Key words
mango/ethylene receptor/gene cloning/sequence analysis分类
农业科技引用本文复制引用
李丽,唐雅园,李杰民,郑凤锦,孙健,易萍,饶川艳,李昌宝,何雪梅,零东宁,肖占仕,盛金凤..芒果乙烯受体ETR1和ERS1基因的克隆及序列分析[J].南方农业学报,2018,49(6):1053-1060,8.基金项目
广西自然科学基金项目(2015GXNSFBA139102) （2015GXNSFBA139102）
广西创新驱动发展专项项目(桂科AA17204038) （桂科AA17204038）
广西农业科学院基本科研业务专项项目(桂农科2015YT86,桂农科2018YM04) （桂农科2015YT86,桂农科2018YM04）
