南方农业学报2018，Vol.49Issue(6)：1061-1067,7.DOI:10.3969/j.issn.2095-1191.2018.06.03
广西百色云耳野生菌株的遗传多样性分析
Genetic diversity of Baise Auricularia auricular wild strains in Guangxi
摘要
Abstract
[Objective]The genetic diversity of Baise Auricularia auricular wild strains in Guangxi was analyzed,in order to provide reference for protection,exploitation and utilization of the resources of Baise A. auricular.[Method]Twenty-three A. auricular wild strains were collected from Baise in Guangxi and artificially cultivated. During this period, their agronomic characters were observed and determined. The polymorphism of esterase isozyme bands in different A. au-ricular strains was analyzed by using esterase isozyme technology,and cluster analysis was also conducted.[Result]Among the 23 wild strains,there were 16 strains with the growth type of individual fruit body,7 strains with clustered fruit body;13 stains had smooth margin and 10 strains had wavy margin;at the back of A. auricular ,15 strains had ob-vious or relatively obvious veins,and 8 strains had no veins or no obvious veins;the surface color of 4 strains was fawn,8 strains tawny,and 11 strains was black brown;the size of surface(length×width)were 30.1-51.6 mm×20.5-41.7 mm and the thickness was 0.95-1.44 mm. These results showed that the morphology characters of Baise A. auricular possessed high diversity. The germination time of primodium varied from 26 to 59 d,and contamination rate of other strains varied from 0 to 51.1%;dry-wet ration of surface varied from 10.1 to 14.1 and yield of first tide varied from 8.2% to 50.5%. These results showed that there were high diversity among different strains on germination time of primordium,anti-weed-moulds,dry-wet ratio and yields. There were 19 bands in total and 18 types of bands detected from the 23 wild strains. The mobility rate(Rf)of bands ranged from 16.6 to 88.6,and the distribution frequency ranged from 4.3% to 100.0%. Three of the bands were the characteristic bands in esterase isozyme bands of Baise A. auricular. The genetic similarity co-efficients ranged from 0.68 to 1.00 among the 23 wild strains. The 23 wild strains could be divided into two groups at the similarity coefficient of 0.68. And the cluster analysis results of most strains were agreed with their geographical distribu-tion.[Conclusion]Baise A. auricular wild strains in Guangxi possesses high genetic diversity,implies many good genetic characters and has great potential for exploitation and utilization.关键词
百色云耳/野生菌株/遗传多样性/农艺性状/酯酶同工酶Key words
Baise Auricularia auricular/wild strains/genetic diversity/agronomic characters/esterase isozyme分类
农业科技引用本文复制引用
吴圣进,陈雪凤,王灿琴,吴小建,韦仕岩,蓝桃菊..广西百色云耳野生菌株的遗传多样性分析[J].南方农业学报,2018,49(6):1061-1067,7.基金项目
广西自然科学基金项目(2017GXNSFAA198292) （2017GXNSFAA198292）
广西农业科学院基本科研业务专项项目(2015YT77) （2015YT77）
广西农业科学院科技发展基金项目(桂农科2017JZ43) （桂农科2017JZ43）
广西特色作物试验站建设项目(桂Ts201415) （桂Ts201415）
