广西师范大学学报（自然科学版）2018，Vol.36Issue(3)：76-79,4.DOI:10.16088/j.issn.1001-6600.2018.03.011
岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究
Electron Microscopy Study on the Apoptosis and Autophagy of the Hepatic Stellate Cells Induced by Total Alkaloids
摘要
Abstract
To study the effects of total alkaloids on apoptosis and autophagy of hepatic stellate cells in vitro.Cell inhibition rates which using different concentrations (0.20、0.25、0.30 g·L―1) of Dehydrocavidine group,Pakistan Martin group and Berberine group to the cultured HSC-T6 cells in vitro were detected.The ultrastructural changes of each group cells were observed by transmission electron microscopy.The results showed that,the cell inhibition rates of HSC-T6 cells induced by different drugs in three groups were dependent on concentrations;the HSC-T6 cells showed typical ultrastructural changes of apoptosis after 24h's processing with different concentrations of the drugs, and there were different levels of the autophagy.Cavitin,Martin and berberine could induce apoptosis and autophagy of the HSC-T6 cells.There three components may be effective to anti liver fibrosis in total alkaloids of the Coptis chinensis Franch.关键词
凋亡/自噬/肝星状细胞/超微结构Key words
apoptosis/autophagy/hepatic stellate cell/ultrastructure分类
医药卫生引用本文复制引用
孟春梅,陆世银,梁永红,莫肖敏,李卫东,黄远洁,成晓静,苏志恒,郑华..岩黄连总碱诱导肝星状细胞凋亡和自噬的电镜实验研究[J].广西师范大学学报（自然科学版）,2018,36(3):76-79,4.基金项目
国家自然科学基金地区基金(81560626) （81560626）
广西自然科学基金面上项目(2013GXNSFAA019161) （2013GXNSFAA019161）
广西高校中青年教师基础能力提升项目(KY2016LX046) （KY2016LX046）
