广西植物2018，Vol.38Issue(6)：818-824,7.DOI:10.11931/guihaia.gxzw201707013
七种风铃草属植物亲缘关系的ISSR分析
ISSR analysis on genetic relationships among seven species in Campanula
摘要
Abstract
Genetic relationships among seven species in Campanula L.were investigated by inter-simple sequence repeat molecular markers (ISSR).The results showed that 28 of 40 primers can be amplified to clear bands.All the 28 ISSR primers amplified 164 bands,and the proportion of polymorphic fragment was 98.8%.The value of genetic similarity (GS) indexes of seven species based on the ISSR data varied from 0.421 to 0.945,and the highest genetic similarity was 0.945 between C.aristata and C.modesta,which indicated that they had closely relative relationship.Cluster analysis showed that all the species could be divided into four groups: C.colorata,C.cana and C.calcicola in the first group,C.calcicola in the second group,C.crenulata in the third group,and C.aristata and C.modesta in the last group.This study provides reference for the molecular marker assisted identification,resource conservation,flower variety develop-ment and breeding of Campanula.关键词
风铃草属/ISSR分子标记/遗传多样性Key words
Campanula L./ISSR molecular marker/genetic diversity分类
农业科技引用本文复制引用
包志远,曲继鹏,杨在君,路璐,廖明莉,周梦霞,彭正松..七种风铃草属植物亲缘关系的ISSR分析[J].广西植物,2018,38(6):818-824,7.基金项目
四川省科技厅应用基础项目(16JC0022) （16JC0022）
西华师范大学英才基金(17YC355) Supported by the Foundation of Science&Technology Department of Sichuan Province(16JC0022) （17YC355）
Talent Fund of China West Normal University(17YC355) （17YC355）
