海南医学院学报2018，Vol.24Issue(12)：1139-1142,4.DOI:10.13210/j.cnki.jhmu.20180424.010
右美托咪定对心肌缺血-再灌注过程中单相动作电位振幅的影响及其与心肌损伤的相关性
Effect of dexmedetomidine on monophasic action potential amplitude in myocardial ischemia-reperfusion and its correlation with myocardial injury
摘要
Abstract
Objective :To study the effect of dexmedetomidine on monophasic action potential amplitude (MAPA) in myocardial ischemia-reperfusion and its correlation with myocardial injury.Methods :SD rats were selected as the experimental animals and randomly divided into control group ,ischemia reperfusion group (I/R group) and dexmedetomidine group (Dex group) ,I/R group and Dex group were made into myocardial ischemia-reperfusion injury models ,and Dex group were given exmedetomidine intervention ;the MAPA of myocardial tunica intima layer ,tunica media layer and tunica externa layer were measured in Langendorff perfusion system ;myocardial tissue was collected to determine the contents of oxidative stress mole-cules and the expression of apoptosis genes.Results :The MAPA levels of myocardial tunica intima layer ,tunica media layer and tunica externa layer as well as Klotho and SOD contents in myocardial tissue of I/R group were significantly lower than those of control group whereas CaMK Ⅱ,NOX2 ,NOX4 and MDA contents as well as CaSR ,USP14 ,JNK ,Bax ,Fas and Caspase-3 mRNA expression in myocardial tissue were significantly higher than those of control group ;the MAPA levels of myocardial tunica intima layer ,tunica media layer and tunica externa layer as well as Klotho and SOD contents in myocardial tissue of Dex group were significantly higher than those of I/R group whereas CaMKⅡ,NOX2 ,NOX4 and MDA contents as well as CaSR ,USP14 ,JNK ,Bax ,Fas and Caspase-3 mRNA expression in myocardial tissue were significantly lower than those of I/R group ;Pearson test showed that the MAPA levels of myocardial tunica intima layer ,tunica media layer and tunica externa layer were negatively correlated with CaMK Ⅱ,NOX2 ,NOX4 and MDA contents as well as CaSR ,USP14 ,JNK , Bax ,Fas and Caspase-3 mRNA expression in myocardial tissue ,and positively correlated with Klotho and SOD contents.Con-clusion :Dexmedetomidine can increase the MAPA in myocardial ischemia-reperfusion process ,and is closely related to the in-hibition of oxidative stress response and apoptosis.关键词
心肌缺血再灌注/右美托咪定/单相动作电位振幅/氧化应激反应/细胞凋亡Key words
myocardial ischemia-reperfusion/dexmedetomidine/monophasic action potential amplitude/oxidative stress response/apoptosis分类
医药卫生引用本文复制引用
蒋明珉,熊丽..右美托咪定对心肌缺血-再灌注过程中单相动作电位振幅的影响及其与心肌损伤的相关性[J].海南医学院学报,2018,24(12):1139-1142,4.基金项目
This study was supported by the 2016 Project of Health and Family Planning Commission of Sichuan Province (16PJ199) （16PJ199）
2016年四川省卫计委课题(16PJ199) （16PJ199）
