海南医学院学报2018，Vol.24Issue(12)：1196-1199,4.DOI:10.13210/j.cnki.jhmu.20180503.005
宫颈癌前病变组织中HPV感染与TLRs通路激活、下游基因表达的相关性
The correlation of HPV infection with TLRs pathway activation and downstream gene ex-pression in cervical precancerous lesions
摘要
Abstract
Objective :To study the correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions.Methods :Cervical precancerous lesion tissues from surgical resection or cone biopsy in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University between June 2014 and May 2017 were col-lected and divided into HR-HPV positive group and HR-HPV negative group according to the condition of high-risk HPV in-fection ;normal cervical tissues surgically removed due to fibroid in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University during the same period were collected as the control group.The contents of TLRs pathway molecules as well as the expression of apoptosis-related genes and invasion-related genes in the cervical precancerous lesion tissues and nor-mal cervical tissues were determined.Results :TLR2 ,TLR3 ,TLR4 ,TLR9 and NF-κB contents as well as CyclinD1 ,PCNA , iNOS ,Gal-9 ,N-cadherin , HIF-1α and VEGF mRNA expression in HR-HPV positive group and HR-HPV negative group were significantly higher than those in control group whereas p53 ,Beclin1 ,LC3-II and SFRP2 mRNA expression were signifi-cantly lower than those in control group (P＜0.05) ,and TLR2 ,TLR3 ,TLR4 ,TLR9 and NF-κB contents as well as Cy-clinD1 ,PCNA ,iNOS ,Gal-9 ,N-cadherin ,HIF-1α and VEGF mRNA expression in HR-HPV positive group were significantly higher than those in HR-HPV negative group whereas p53 ,Beclin1 ,LC3-II and SFRP2 mRNA expression were significantly lower than those in HR-HPV negative group (P＜0.05).Conclusion :HPV infection in cervical precancerous lesions can regu-late downstream apoptosis and invasion gene expression by activating multiple TLRs pathways.关键词
宫颈癌/癌前病变/高危型人乳头瘤病毒/Toll样受体Key words
cervical cancer/precancerous lesion/high-risk human papillomavirus/Toll-like receptor分类
医药卫生引用本文复制引用
比丽克孜·艾克木,龚巍,巴哈尔古丽·依斯巴依力,岳明明..宫颈癌前病变组织中HPV感染与TLRs通路激活、下游基因表达的相关性[J].海南医学院学报,2018,24(12):1196-1199,4.基金项目
It is supported by Xinjiang Natural Science Foundation Project (2015211C150) （2015211C150）
新疆维吾尔自治区自然科学基金资助项目(2015211C150) （2015211C150）
