河南农业大学学报2018，Vol.52Issue(3)：356-360,5.
猪细小病毒NS1基因真核表达载体pEGFP-N1-NS1的构建与表达
Construction and expression of eukaryotic expression vector pEGFP-N1-NS1 for porcine parvovirus NS1 gene
摘要
Abstract
According to the sequences of PPV genes in the GenBank,the special primers for non-structural protein NS1 gene were designed.The NS1 gene of PPV virus was amplified by PCR and cloned into pEGFP-N1 vector to construct a plasmid,named pEGFP-N1-NS1.The plasmids were identified by double enzyme digestion and sequencing.The correcting plasmids were transfected into 293T cells and identified by fluorescence and PCR methods.The results showed that the recombinant eukaryotic expression vector pEGFP-N1-NS1 was successfully constructed; and the NS1 protein was finally expressed in 293T cells.关键词
猪细小病毒:真核载体/NS1Key words
porcine parvovirus virus/eukaryotic vector/NS1分类
农业科技引用本文复制引用
周雍,张驰,王朋涛,韩丽,靳晓慧,魏战勇..猪细小病毒NS1基因真核表达载体pEGFP-N1-NS1的构建与表达[J].河南农业大学学报,2018,52(3):356-360,5.基金项目
国家自然科学基金项目(U140410985) （U140410985）
河南省高校科技创新人才项目(15HASTIT028) （15HASTIT028）
