河南农业大学学报2018，Vol.52Issue(3)：361-365,5.
猪源铁蛋白重链亚基纳米粒的制备
Preparation of swine ferritin heavy-chain subunit nanoparticles
摘要
Abstract
This paper was to study a nanoparticle preparation for swine ferritin sub-units.Firstly,a pair of specific primers for the ferritin heavy chain gene were designed,whose sequence was referred on the GenBank website.Total RNA was extracted from the porcine heart sample,and the gene segment for the H-chain subunit was amplified by RT-PCR.The verified gene segment was integrated into the PET32a vector,and this recombinant plasmid was similarly clarified by PCR amplification,dual-enzyme digestion,and sequencing.The plasmid was then transformed into E.coli BL21(DE3)and protein expression was induced by isopropyl-β-D-thiogalactoside(IPTG).The expressed recombinant protein was purified by Ni+ion affinity chromatography,and identified by SDS-PAGE and Western blot.Transmission electron microscopy(TEM)was employed to visualize the size of protein.The results showed that the ferritin H-chain subunit was successfully expressed by E.coli BL21,and particles with diameter range of 10nm and 13 nm were observed.It indicated that the nanoparticles of swine ferritin sub-units were successfully obtained assemblied.关键词
猪/铁蛋白/纳米粒/免疫佐剂Key words
Sus scrofa/ferritin/nanoparticles/immune-adjuvant分类
农业科技引用本文复制引用
李滨洲,和春昊,吴秋玲,郭珍珍,张留君,杨国宇,王亚宾,陈丽颖..猪源铁蛋白重链亚基纳米粒的制备[J].河南农业大学学报,2018,52(3):361-365,5.基金项目
河南省重点科技攻关项目(152102310081). （152102310081）
