河南农业大学学报2018，Vol.52Issue(3)：412-417,423,7.
表面活性剂降低烟碱对纤维素酶活性抑制作用的研究
Effects of reducing nicotine on cellulase activity by surfactants
摘要
Abstract
In order to obtain the efficient method of tobacco enzymolysis this study examined the effects of reduing nicotine on cellulase activity inhibition by surfactant.Different surfactants including PEG 6000,Tween 80,BSA and Rhamnolipid were used to reduce nicotine production,subsequently allevi-ate the cellulase activity.The results showed that surfactants on the other hand,could alleviate the in-hibitory effect of nicotine on the activity of cellulase,0.1％nicotine could remarkably decrease the ac-tivity of cellulase activity.If the fraction of nicotine was increased,the inhibitory effects of nicotine on cellulase activity was significantly enhanced.Compare to control,when 0.001 g·g-1 of BSA and 0.03 g·g-1 of Tween 80 were added to the substrate,total reducing sugar concentration of tobacco stalk hydrolysate was increased by 13.70％and 10.34％,respectively.Compared to the control respectively.While hemicellulose conversion rate were increased by 50.94％and 40.04％with 0.03 g·g-1 of Tween 80 and 0.001 g·g-1 of BSA,respectively.But there was no obviously change in cellulose con-version rate.关键词
烟碱/木质纤维素/表面活性剂/酶解Key words
nicotine/lignocellulose/surfactant/enzymatic hydrolysis分类
化学化工引用本文复制引用
王风芹,苏增平,付晨青,谢慧,毛国涛,任天宝,杨森,宋安东..表面活性剂降低烟碱对纤维素酶活性抑制作用的研究[J].河南农业大学学报,2018,52(3):412-417,423,7.基金项目
河南省高校科技创新团队支持计划(15IRTSTHN014) （15IRTSTHN014）
