河南农业大学学报2018，Vol.52Issue(3)：418-423,6.
烟叶中纤维素高效降解菌的筛选与作用效果研究
Study on screening and application of an efficient cellulose-degrading strain from tobacco leaf
摘要
Abstract
In order to improve the tobacco quality by degrading the cellulose,several cellulose-degrading strains were isolated from the surface of tobacco leaves.The activity of cellulose was detected,and the strain with the highest cellulase-producing activity was identified by the 16 S rDNA gene sequence and physiological-biochemical tests.Orthogonal experiment was further designed to get the optimal fermentation conditions of cellulose degrading in the tobacco leaves.The results showed that:(1)strain XC-19-1 had the highest cellulase enzyme producing activity(6.6 U·mL-1)among all eleven isolated strains;(2)strain XC-19-1 was identified as Bacillus subtilis by the 16 S rDNA gene sequence and physiological-biochemical tests;(3)the orthogonal experiment result suggested that,by adding 3％culture medium(with strain XC-19-1)to the surface of tobacco leaf,the degrading rates of cellulose could reach 30.6％after 60 hours fermentation under the condition of 37 ℃ temperature and 75％humidity.The contents of total sugar and reducing sugar were both increased after fermentation,which indicated the improvement of tobacco quality.关键词
烟叶/纤维素降解/16S rDNA测序/枯草芽孢杆菌Key words
tobacco/cellulose degradation/16 S rDNA-gene sequence/Bacillus subtilis分类
农业科技引用本文复制引用
杨宗灿,冯颖杰,李龙飞,张耀广,李家美,刘超..烟叶中纤维素高效降解菌的筛选与作用效果研究[J].河南农业大学学报,2018,52(3):418-423,6.基金项目
河南中烟科技创新项目(ZW2014035) （ZW2014035）
