化工进展2018，Vol.37Issue(7)：2798-2805,8.DOI:10.16085/j.issn.1000-6613.2017-1738
仿生合成氧化锆固定化漆酶处理孔雀石绿
Treatment of malachite green by immobilized laccase in ZrO2 via biomineralization
摘要
Abstract
A method of laccase immobilization was developed by ZrO2 synthesized via biomineralization, which was prepared by encapsulating laccase in the zirconia nanoparticles with the lysozyme as the inducer, and the recovery rate of enzyme activity was 59%. Field emission scanning electron microscope (FESEM), energy dispersive spectrometer (EDS), and thermal gravimetric analyzer (TGA) were used to characterize ZrO2 nanoparticles before and after the enzyme immobilization. The results proved that laccase was immobilized in ZrO2 successfully and lysozyme was not only induced the formation of zirconia but also acted as a biological template encapsulated by ZrO2. Enzymatic properties of immobilized laccase by biomimetic synthesis were also explored. The optimum pH and temperature of immobilized laccase were 3 and 70℃, respectively. Compared with free enzyme, the pH and temperature stability of immobilized laccase were significantly increased. The activity of the immobilized laccase was 95% of the original activity after storing for 30 days at 4℃, and the activity was still 60% of the initial enzyme activity after recycling 5 times. Moreover, the immobilized laccase was used in the decolorization of malachite green and the experimental result showed that the decolorization rate of the malachite green was more than 95% within 6h. Additionally it proved that the immobilized laccase decolorized malachite green by both of adsorption and degradation effects through the UV-visible spectroscopy analysis.关键词
仿生合成/氧化锆/酶/固定化/纳米材料/孔雀石绿Key words
biomemitic synthesis/zirconium oxide/enzyme/immobilization/nanomaterials/malachite green分类
资源环境引用本文复制引用
王美银,张新颖,林琳琳,李小龙,李宗仁..仿生合成氧化锆固定化漆酶处理孔雀石绿[J].化工进展,2018,37(7):2798-2805,8.基金项目
省部共建能源与环境光催化国家重点实验室自主课题项目(2014C05). （2014C05）
