化工学报2018，Vol.69Issue(6)：2627-2631,5.DOI:10.11949/j.issn.0438-1157.20171414
重组大肠杆菌全细胞用于苯乙酮酸的绿色生物合成
Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells
摘要
Abstract
Phenylglyoxylic acid (PGA) is key building block in the chemical synthesis, which could be used to synthesize a variety of important pharmaceutical intermediate, thus exploiting the green synthesis process of phenylacetone acid has significant economic value. The recombinant Escherichia coli whole cells containing the encoding gene of LhDMDH was used as catalyst in this study. The biotransformation of D-mandelic acid was researched under the condition of no coenzyme and cosubstrate addition. Subsequently, the transformed products were purified and identified. These results indicated that biosynthesis of phenylglyoxylic acid was realized under the condition of no coenzyme and cosubstrate addition. The yield and purity of PGA were 45% and 99%, respectively. In addition, this study established a solid foundation for chiral resolution of racemic mandelic acid and the biosynthesis of PGA.关键词
扁桃酸脱氢酶/苯乙酮酸/全细胞催化/绿色化学/生物转化Key words
mandelate dehydrogenase/phenylglyoxylic acid/whole cell catalysis/green chemistry/biotransformation分类
化学化工引用本文复制引用
唐存多,史红玲,和子涵,丁朋举,焦铸锦,阚云超,姚伦广..重组大肠杆菌全细胞用于苯乙酮酸的绿色生物合成[J].化工学报,2018,69(6):2627-2631,5.基金项目
河南省科技攻关项目(162102210116) （162102210116）
南阳师范学院青年项目(17359) （17359）
河南省科研服务平台专项资助(2016151) （2016151）
河南省南水北调中线水源区水生态安全创新型科技团队专项(17454) （17454）
河南省科技厅基础与前沿项目(162300410137) （162300410137）
河南省高等学校重点科研项目(16A2100347). （16A2100347）
