湖南大学学报（自然科学版）2018，Vol.45Issue(6)：113-119,7.DOI:10.16339／j.cnki.hdxbzkb.2018.06.017
PHBV/葡聚糖纳米药物载体的制备及表征*
Fabrication and Characterization of Novel PHBV/dextran Nanoparticles Drug Delivery System
摘要
Abstract
Amphiphilic polymer nanoparticles have attracted considerable attention especially as anti-cancer drug delivery system,since they can enhance bioavailability and reduce side effects of drugs used for chemotherapy.Novel poly(3-hydroxybutyric-co-3-hydroxyvaleric,PHBV)/dextran nanoparticles(PD-NPs)drug delivery system was formulated via an original double emulsion(w1/o1/w2)solvent-evaporation method.The mean diameter of PDNPs was 205.0±6.9 nm and their zeta potential was-1.59±0.12 mV by using dynamic light scattering(DLS)detection.PDNPs showed obvious core-shell structure with uni-form particle dispersion.Cisplatin,as a model anticancer drug,was loaded to demonstrate the character-izations of the drug delivery system,and its loading did not significantly alter the hydrodynamic diameter and zeta potential of nanoparticles with 19.3±2.9％of drug loading content(DLC).In-vitro drug release profile of cisplatin from cisplatin-loaded PDNPs found that it can be released faster in normal cells(pH=7.4)than cancer cells(pH=5.5)in phosphate buffered saline(PBS),and the release period was more than 7 d,which demonstrated that this drug delivery system was pH-responsive and with excellent slow-re-lease performance.PDNPs displayed no cytotoxicity,and cisplatin-loaded PDNPs exhibited higher killing efficient to cancer cells when compared with normal cells,which indicated this nano-delivery system can kill cancer cells more effectively.Thus,the novel PDNPs drug delivery system can be potentially applied for cancer chemotherapy,which will improve the utilization ratio and reduce side effect of drugsin the canc-er chemotherapy.关键词
PHBV/葡聚糖/纳米颗粒/药物载体/体外药物释放/细胞毒性Key words
PHBV/dextran/nanoparticles/drug delivery system/drug release in vitro/cytotoxicity分类
医药卫生引用本文复制引用
刘海蓉,张清卿,周征,胡薏冰,张水寒,戴瑶,李永生..PHBV/葡聚糖纳米药物载体的制备及表征*[J].湖南大学学报（自然科学版）,2018,45(6):113-119,7.基金项目
湖南省自然科学基金资助项目(2016JJ2023),National Natural Science Foundation of Hunan Province(2016JJ2023) （2016JJ2023）
湖南省科技计划项目(2015TP1035) （2015TP1035）
湖南省研究生创新基地(2014-2017) （2014-2017）
湖南大学交叉学科研究项目(2014JCA09) （2014JCA09）
