华南农业大学学报2018，Vol.39Issue(4)：1-6,6.DOI:10.7671/j.issn.1001-411X.2018.04.001
3种免疫增强剂对猪伪狂犬灭活疫苗的免疫效果评价
Evaluation of the immune effects of three immunopotentiators on pseudorabies virus inactivated vaccine
摘要
Abstract
[Objective]To evaluate the immunopotentiation effects of levamisole hydrochloride(LH),concanavalin A(ConA)and muramyl dipeptide(MDP)on pseudorabies virus(PRV)inactivated vaccine.[Method]The immunopotentiators were mixed into inactivated PRV virus aqueous phase,and emulsified with oil phase to make inactivated vaccines containing immunopotentiators.The final concentrations of immunopotentiators were LH 7.5 mg·mL–1,ConA 25.0 mg·mL–1and MDP 150.0 mg·mL–1respectively.The second immunization was on the 14th day after the first immunization.Mouse serum samples were collected on the 14th day after the first immunization,7th and 14th day after the second immunization to detect relative cytokines(IL-2,IL-4,IFN-γ)and specific antibody levels.On the 14th day after the second immunization,all mice were attacked and the final attack protective rate was calculated.[Result]Compared to Baiyou group,ConA and MDP groups extremely significantly improved IL-2 content in mouse serum on the 14th day after the second immunization.LH group significantly improved IL-4 content on the 14th day after the first immunization,and MDP group significantly improved IL-4 content on the 14th day after the second immunization.The three immunopotentiators extremely significantly enhanced IFN-γ contents and specific antibody levels on the 14th and 7th day after the second immunization respectively,and also improved the attack protective rates.The survival rates of LH and ConA groups were 20％,while that of MDP group was 60％.[Conclusion]Three immunopotentiators within the range of the experimental dosages can enhance the immune effects of PRV inactivated vaccine in varying degrees.关键词
免疫增强剂/猪伪狂犬灭活疫苗/免疫增强效果/攻毒保护率Key words
immunopotentiator/pseudorabies virus inactivated vaccine/immune enhancement effect/attack protective rate分类
农业科技引用本文复制引用
邹舒展,李冰,陈安妮,吴佳骏,姜增,罗满林..3种免疫增强剂对猪伪狂犬灭活疫苗的免疫效果评价[J].华南农业大学学报,2018,39(4):1-6,6.基金项目
广东省科技计划项目(2013B020307010) （2013B020307010）
华南农业大学与广东大华农动物保健品股份有限公司技术开发项目(h2015415) （h2015415）
