华南农业大学学报2018，Vol.39Issue(4)：13-19,7.DOI:10.7671/j.issn.1001-411X.2018.04.003
杂交鲟鲁氏耶尔森氏菌的分离鉴定及感染的病理损伤
Isolation and identification of Yersinia ruckeri from hybrid sturgeon and pathological lesions of its infection
摘要
Abstract
[Objective]To explore the etiology of a serious infectious disease occured in hybrid sturgeon(Acipenser schrencki♀×A.baeri♂)from a farm of Qionglai,Sichuan Province.[Method]The pathogens were isolated from the liver,spleen and kidney tissues of diseased fish,and identified by physicochemical characteristics,16S rRNA and gyrB gene sequences analyses,meanwhile the susceptibility test and histopathological observation were performed.[Result]One gram-negative predominant bacteria strain(YC160412)was isolated and identified as Yersinia ruckeri by physicochemical characteristics,16S rRNA and gyrB gene sequences analyses.The antibiotic susceptibility test showed that the pathogen was sensitive to cefazolin,ampicillin,florfenicol,and resistant to amoxicillin and erythromycin.Histopathological observation found that Y.ruckeri infection caused significant damages to hybrid sturgeon multiple organs,especially in liver,spleen,kidney,intestine and gill.The main pathologic lesions were obvious degeneration,necrosis,hemorrhage and infiltration of the inflammation cells.The pathogen was largely found in liver,spleen and kidney tissues.[Conclusion]This study confirms Y.ruckeri is the pathogen of the epidemic.Its infection can cause multiple organ dysfunctions and even death of hybrid sturgeon.关键词
杂交鲟/鲁氏耶尔森氏菌/分离鉴定/病理损伤Key words
hybrid sturgeon/Yersinia ruckeri/isolation and identification/pathological lesion分类
农业科技引用本文复制引用
曹师琪,耿毅,余泽辉,郑李平,雷雪平,陈德芳,欧阳萍,黄小丽,黄彦臻..杂交鲟鲁氏耶尔森氏菌的分离鉴定及感染的病理损伤[J].华南农业大学学报,2018,39(4):13-19,7.基金项目
四川省淡水鱼产业技术体系创新团队建设项目(2017SICAD002) （2017SICAD002）
四川省重点研发项目(18ZDYF) （18ZDYF）
