华南农业大学学报2018，Vol.39Issue(4)：20-24,5.DOI:10.7671/j.issn.1001-411X.2018.04.004
温度对锦鲤疱疹病毒体外培养和致病性的影响
Effects of temperature on culture in vitro and pathogenicity of Cyprinid herpesvirus 3
摘要
Abstract
[Objective]To confirm the effect of temperature on proliferation in vitro and pathogenicity of Cyprinid herpesvirus 3(CyHV-3).[Method]CyHV-3-EGFP strain was used in this study.Viral titer was measured by the indirect immunofluorescence staining assay.The proliferation of virus in vitro was observed using a fluorescence microscope.The thermal stability,and the effect of temperature on proliferation in vitro and pathogenicity in vivo of virus were studied.[Result]CyHV-3 had good thermal stability at 4-36℃.The viability of CyHV-3 decreased with the increase of temperature when the temperature exceeded 36 ℃.The virus viability was completely lost at 50 ℃.The infection temperature had little effect on the viability of CyHV-3,and the virus showed infectivity at both high(37 ℃)and low(4 ℃)temperatures.However,incubation temperature significantly affected the virus viability.CyHV-3 proliferation capacity decreased or even disappeared when the temperature exceeded 30 ℃.After CyHV-3 infection,the mortality rates for the carps at low temperature(15 ℃)and room temperature(25 ℃)were 26.67％and 73.33％respectively,and the mortality rates for the carps at high temperature(30 ℃)and in the control group with no virus infection were 0.[Conclusion]The results confirm that CyHV-3 can proliferate and has pathogenicity at low temperature,which explain the phenomenon that the outbreak and rapid spread of KHVD in recent years are no longer restricted in spring and autumn seasons.关键词
锦鲤疱疹病毒Ⅲ型/鲤鱼/锦鲤疱疹病毒病/温度/致病性/增殖/感染Key words
Cyprinid herpesvirus 3/Cyprinus carpio/koi herpesvirus disease/temperature/pathogenicity/proliferation/infection分类
农业科技引用本文复制引用
周瑶佳,涂尊方,税斐,阳瑞雪,汪开毓,耿毅,黄小丽,欧阳萍..温度对锦鲤疱疹病毒体外培养和致病性的影响[J].华南农业大学学报,2018,39(4):20-24,5.基金项目
中国博士后科学基金(2015M582563) （2015M582563）
教育部留学回国人员科研启动基金(教外司留[2015]311号) （教外司留[2015]311号）
