湖南中医药大学学报2018，Vol.38Issue(3)：250-253,4.DOI:10.3969/j.issn.1674-070X.2018.03.005
美洲大蠊提取液对慢性难愈合创面I/Ⅲ型胶原蛋白调控研究
Study of American Cockroach Extraction Solution in Regulating the Type I /Ⅲ Collagen of Chronic Wound
摘要
Abstract
Objective To observe the effect of American Cockroach extraction solution on the expression of type I /Ⅲ collagen in tissue of the chronic wound in rats, and to investigate the healing mechanism. Methods The 48 SPF rats were randomly divided into the acute wound control group (control group), the chronic wound model group (model group), the positive group and the experimental group. The research group kept a part of wound tissue in batches on 5th, 7th, 11th day after drug intervention and executed the whole rats finally. Expression quantity of type collagen I /Ⅲ mRNA in wound tissue was measured by polymerase chain reaction (PCR). Results The curves of expression variation of collagen type I and Ⅲ were consistent in each group. On the 5th day, the expression quantity in the control group was highest and the model group's was lowest. Collagen type I/Ⅲ of the experimental group was on the rise from the 5th day and it was higher than that of positive group, it kept steadily after reaching the peak on the 7th day,and it was slightly lower than that of the positive group on 11st day (P<0.0l). Conclusion American cockroach extraction solution could improve the expression of type I /Ⅲ collagen of skin tissue in the middle time of the chronic wound healing of rats effectively, and it could reduce the excessive expression of collagen effectively so as to reduce the scar formation.关键词
美洲大蠊提取液/慢性难愈合创面/I型胶原蛋白/Ⅲ型胶原蛋白Key words
American cockroach extraction solution/chronic wound/type I collagen/type Ⅲ collagen分类
医药卫生引用本文复制引用
李文华,伍伟明,刘筱,杨敏,周雯婷,曾娟妮..美洲大蠊提取液对慢性难愈合创面I/Ⅲ型胶原蛋白调控研究[J].湖南中医药大学学报,2018,38(3):250-253,4.基金项目
国家自然科学基金项目(81503585). （81503585）
