湖南中医药大学学报2018，Vol.38Issue(3)：254-256,3.DOI:10.3969/j.issn.1674-070X.2018.03.006
小檗碱体外抗耐甲氧西林金黄色葡萄球菌活性的研究
Effect of Berberine on the Activity of Methicillin-Resistant Staphylococcus aureus in Vitro
摘要
Abstract
Objective To study berberine on the activity of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Methods The size of antibacterial annulus, minimum inhibitory concentration (MIC) and minimum bactericidal concentration(MBC) of MRSA intervened by berberine were measured by Agar diffusion test and broth dilution test. The crystal violet staining method measured on 96 well Elisa-plate was applied to analyze minimal biofilm eradication concentration (MBEC). Results Compare with DMSO,berberine and chloramphenicol (positive control drug) could significantly increase the antibacterial annulus radius of MRSA (P<0.0l). The MIC, MBC and MBEC of berberine against MRSA were 0.2 mg/mL, 0.4 mg/mL and0.2 mg/mL, respectively. Conclusion Berberine shows obvious inhibition effect on MRSA, and its mechanism may be related to the inhibition of bacterial biofilm formation.关键词
小檗碱/耐甲氧西林金黄色葡萄球菌/生物膜Key words
berberine/methicillin-resistant Staphylococcus aureus/biofilm分类
医药卫生引用本文复制引用
李玲,卢芳国,陈伶利,向琴,高强,魏科,胡珏,宁毅..小檗碱体外抗耐甲氧西林金黄色葡萄球菌活性的研究[J].湖南中医药大学学报,2018,38(3):254-256,3.基金项目
国家自然科学基金资助项目(81473468,81503445,81774126,81703919) （81473468,81503445,81774126,81703919）
湖南省教育厅创新平台开放基金项目(17K067) （17K067）
湖南省自然科学基金(2016JJ2095,2016JJ3098) （2016JJ2095,2016JJ3098）
湖南省教育厅优秀青年基金(15B169) （15B169）
湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目(15) （15）
湖南省省级精品课程《免疫学基础与病原生物学》资助项目(48) （48）
湖南中医药大学重点学科《基础医学》、《病原生物学》资助项目(1). （1）
