湖南中医药大学学报2018，Vol.38Issue(3)：257-260,4.DOI:10.3969/j.issn.1674-070X.2018.03.007
补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后Bcl-2、Bax表达的影响
Effects of Buyang Huanwu Ultra-Fine Powder on Apoptosis Gene Bcl-2 and Bax of Transplanted Neural Stem Cells in Rats with Focal Cerebral Ischemia
摘要
Abstract
Objective To investigate the effects of Buyang Huanwu ultra-fine powder on apoptosis protein of Bcl-2 and Bax in transplanted neural stem cells in rats with focal cerebral ischemia. Methods Eighty Sprague-Dawley rats, half male and half female, were copyed the focal cerebral ischemia models, which were randomly divided into five groups, 15 rats in each group. The model rats were intervened by using Buyang Huanwu ultra-fine powder and traditional decoction. At 7 d,14 d and 28 d of post-tansport, the neural function defect scale was measured. The rats were sacrified at according time. Bcl-2 and Bax were detected by immunohistochemical staining. Results Buyang Huanwu decoction could improve the neurological defect Compared with model group, the expression of Bcl-2 and Bax increased obviously at different time points(7 d,14 d, 28 d),the differnces were statistically significant (P<0.05). The model-transplantation-Buyang Huanwu ultra-fine powder group in increasing Bcl-2 and decreasing Bax was better than the model-transplantation-traditional Buyang Huanwu decoction, the difference was statistically significant (P<0.05). Conclusion Buyang Huanwu decoction could regulate the expression of Bcl-2 and Bax protein after cerebral ischemia, which may be one of mechnisms of promoting the recovery of neural function. The effect of Buyang Huanwu ultra-fine powder was better than traditional decoction.关键词
补阳还五汤/局灶性脑缺血/神经干细胞/细胞移植/Bcl-2/BaxKey words
Buyang Huanwu decoction/cerebral ischemia/neural stem cell/cell transplantation/Bcl-2/Bax分类
医药卫生引用本文复制引用
廖亮英,蔡光先,周赛男..补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后Bcl-2、Bax表达的影响[J].湖南中医药大学学报,2018,38(3):257-260,4.基金项目
国家自然科学基金(81302953) （81302953）
湖南省中医药科研计划项目(2014136) （2014136）
湖南省教育厅科研项目(15C1050) （15C1050）
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201510). （ZYFT201510）
