湖南中医药大学学报2018，Vol.38Issue(3)：275-279,5.DOI:10.3969/j.issn.1674-070X.2018.03.011
补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究
Effects of Bushen Huoxue Decoction on β-catenin Protein Level of WNT Signal Pathway in Lumbar Intervertebral Disc Degeneration Aging Model Rats
摘要
Abstract
Objective To observe the effect of Bushen Huoxue decoction on the structural and histopathological changes and β-catenin protein in lumbar intervertebral disc degeneration aging model rats. Methods Eighty three-month old SD rats(male-female half and half) were randomly divided into blank control group, sham-model group, model group, TCM low-dose group and TCM high-dose group, 16 rats in each group. Except the blank group and sham-model group, the aging model rats in other groups were established by injecting D-galactose. The rats in blank control group,sham-model group and model group were daily given normal saline by gavage. TCM low-dose and high-dose groups were daily treated with Bushen Huoxue decoction for 12 weeks by gavage. Two male rats and two female rats in each group were randomly executed at after modelling, after 4 weeks of gavage, after 8 weeks of gavage and after 12 weeks of gavage, and samples were selected from dead rats. The histomorphological change of model group was observed by light microscope, and the expression of β-catenin protein was detected by Western Blot method. Results Compared with the model group, the histomorphology score in TCM low-dose and high-dose groups treated with Bushen Huoxue decoction decreased (P<0.05), and the expression of β-catenin protein decreased (P<0.05). Conclusion Buyang Huanwu decoction shows inhibition effect on the expression of key β-catenin protein in the classical Wnt/β-catenin signal pathway, and thus could relieve the degeneration of intervertebral disc.关键词
补肾活血汤/腰椎间盘退变/β-catenin蛋白Key words
Bushen Huoxue decoction/lumbar intervertebral disc degeneration/β-catenin protein分类
医药卫生引用本文复制引用
谢芳,吴官保,姚红艳,冯帅华,杨届..补肾活血汤干预衰老大鼠退变椎间盘模型中经典WNT信号通路β-catenin蛋白含量的研究[J].湖南中医药大学学报,2018,38(3):275-279,5.基金项目
湖南省中医药管理局资助项目(201221). （201221）
