华侨大学学报（自然科学版）2018，Vol.39Issue(4)：583-588,6.DOI:10.11830/ISSN.1000-5013.201711035
单环刺螠纤溶酶的季节积累及初步药效学
Seasonal Accumulation of Monocyclic Thrips Fibrinolytic Enzyme UFE and Preliminary Pharmacodynamic Study of UFE
摘要
Abstract
The season cumulative rules of the monocyclic sting fibula plasmin was studied,and the pharmaco-dynamics of U rechis unicinctus fibrinolysin enzymes (UFE)was verified.The mononuclear stinger fluid col-lected in different seasons was separated and purified by centrifugation,dextrane gel filtration and anion ex-change chromatography to obtain single component.And then,the relative molecular weight of UFE was de-termined by polyacrylamide gel electrophoresis (SDS-PAGE),the activity of UFE was measured by Folin-phe-nol reagent method,and the anticoagulant effect and thrombolytic effect of UFE was evaluated by in vivo and in vitro thrombolysis tests and arterial embolism model in rat brain.The results showed that the specific activ-ity of UFE isolated and purified from M onocha m us alternatus in autumn was 11.672 μkat·mg-1,the purifica-tion fold was 17.9 folds,and the recovery rate was 16.9%,which was significantly different from the spring batch and summer batch results.Pharmacodynamics experiments confirmed that UFE was similar to uroki-nase,which could prolong the clotting time in vitro significantly,and inhibit thrombosis in vivo effectively.In addition,the content of UFE was related to the seasonal change and had season accumulation.Besides,UFE had a significant anticoagulant and thrombolytic effect with no hemolysis toxicity.关键词
单环刺螠/单环刺螠纤溶酶/抗凝血/溶栓/季节累积性/药效学Key words
Urechis unicinctus /Urechis unicinctus fibrinolysin enzymes/anticlotting/thrombolytic/seasonal accumulation/pharmacodynamics分类
医药卫生引用本文复制引用
龙莎,吕卓伦,李小红,宋伟杰,王立强..单环刺螠纤溶酶的季节积累及初步药效学[J].华侨大学学报（自然科学版）,2018,39(4):583-588,6.基金项目
国家重点研发计划资助项目(2016YFE0101700) （2016YFE0101700）
福建省自然科学基金资助项目(2015J01342) （2015J01342）
华侨大学研究生科研创新基金资助项目(1611316003) （1611316003）
