基础医学与临床2018，Vol.38Issue(7)：950-956,7.
微粒体谷胱甘肽S转移酶1过度表达促进肝癌发生发展
MGST1 overexpression promotes hepatocellular carcinoma development
摘要
Abstract
Objective To investigate the expression of microsomal glutathione S-transferase 1 ( MGST1) in hepa-tocellular carcinoma ( HCC) and its significance in the development of HCC. Methods Western blot was used to measure MGST1 expression in human hepatocellular carcinoma and adjacent tissues and HCC cell lines. Further-more, shRNA targeting MGST1 was constructed and transected into MHCC97H and HCCLM3 cells to deplete MGST1 expression. MGST1 was over-expressed in SK-Hep-1 cells using pCDH lentivirus system. Cell proliferation and migration were analyzed by colony formation and Transwell migration assay, respectively. The subcutaneous xenograft model of MHCC97H cells in nude mice was established to check tumor development and mouse survival.Results MGST1 was higher in 71% (17/24) of HCC tissues compared with their adjacent liver tissues. Cell proliferation and migration were significantly decreased by MGST1 knockdown, while they were increased by MGST1 overexpression. Furthermore, mice implanted with shMGST1 MHCC97H cells exhibited retarded tumor formation and tumor progression compared with control group. Conclusions MGST1 overexpression promotes hepatocellular carcinoma development and this molecule targeted for HCC treatment.关键词
肝癌/MGST1/裸鼠Key words
hepatocellular carcinoma/MGST1/nude mice分类
医药卫生引用本文复制引用
蔡培,黄富强,赵齐,周贤,杨华瑜,毛一雷,张宏冰..微粒体谷胱甘肽S转移酶1过度表达促进肝癌发生发展[J].基础医学与临床,2018,38(7):950-956,7.基金项目
国家自然科学基金重点项目(81730078) （81730078）
