解放军医学院学报2018，Vol.39Issue(1)：65-68,81,5.DOI:10.3969/j.issn.2095-5227.2018.01.018
敲低CUEDC2基因表达可增强卵巢癌细胞对顺铂的敏感性
CUEDC2 down-regulation enhanced sensitivity of ovarian carcinoma cells to cisplatin
摘要
Abstract
Objective To investigate the effect of CUE domain-containing protein 2 (CUEDC2) on the sensitivity of ovarian cancer cellstocisplatin and its mechanisms. Methods CUEDC2 gene in SKOV3 cells were knocked down by siRNA, and then MTS assay and Trypan blue staining technique were used to detect the effect of cisplatin on the cell proliferation of si-control group's (treated with si-control 50 nmol/L) and Si-CUEDC2 group's (treated with Si-CUEDC2 50 nmol/L) SKOV3 cells. SKOV3 cells were grouped into drug group (cisplatin 8 μ mol/L), si-control group (cisplatin 8 μ mol/L, si-control 50 nmol/L), Si-CUEDC2 group (cisplatin 8 μ mol/L, Si-CUEDC2 50 nmol/L), non-treatment group, and the expressions of related protein in MAPK were evaluated by western blotting. Results There was no significant difference in the quantity of SKOV3 cells between Si-CUEDC2 without cisplatin treatment group and si-control group. Compared with si-control group, the quantity of SKOV3 cells decreased significantly in the Si-CUEDC2 with cisplatin treatment group (P ＜ 0.05). Under the effect of cisplatin, p38 phosphorylation level increased while p-ERK phosphorylation level decreased in SKOV3 cells with knockdown of CUEDC2. Conclusion Knocking down CUEDC2 can increase the sensitivity of SKOV3 cells to cisplatin, and CUEDC2 may play a role in regulating the effect of cisplatin on ovarian cancer cell proliferation through MAPK pathway.关键词
CUEDC2蛋白/卵巢癌/促分裂素原活化蛋白激酶通路/顺铂Key words
CUEDC2/ovarian carcinoma/mitogen-activated protein kinase/cisplatin分类
医药卫生引用本文复制引用
李金航,王爱春,周涛,李涛,蔡宏,刘爱军..敲低CUEDC2基因表达可增强卵巢癌细胞对顺铂的敏感性[J].解放军医学院学报,2018,39(1):65-68,81,5.基金项目
部委级资助项目 （）
解放军总医院临床科研扶持基金(2016F-C-CXYY-2012) （2016F-C-CXYY-2012）
