解放军医学院学报2018，Vol.39Issue(2)：140-143,149,5.DOI:10.3969/j.issn.2095-5227.2018.02.014
五味苦参肠溶胶囊对溃疡性结肠炎小鼠半胱天冬氨酸蛋白酶1、白细胞介素-1β蛋白的影响
Effects of composite sophora colon-soluble capsules on level of caspase-1, IL-1 β in mice with ulcerative colitis
摘要
Abstract
Objective To observe the effect of composite sophora colon-soluble capsules (CSCC) on expressions of caspase-1 and interleukin 1-beta (IL-1 β) in colon tissues of mice with ulcerative colitis (UC) induced by dextran-sulfate-sodium (DSS). Methods Fifty BALB/C mice were randomly divided into five groups: normal control group (Nctrl group), model control group (Model group), low dose of CSCC group [1.92 g/(kg·d)], moderate dose of CSCC group [3.84 g/(kg·d)], and high dose of CSCC group [7.68 g/(kg·d)], with ten mice in each group. After drinking water with 3％ DSS for seven days in all groups except Nctrl, Nctrl group and model group received 0.9％ saline enema for seven days, while CSCC groups received CSCC suspension enemas at different concentration respectively as mentioned before. The pathology of colon tissues were observed, and the length of colon, the coefficient of colon and spleen were measured. Then the pathological changes of colon tissues were stained by HE staining and observed under microscope, and the protein levels of Caspase-1 and IL-1 β in colon tissues were detected by ELISA. Results Compared with Nctrl group, disordered colon tissue structure, decrease of goblet cell, infiltrated inflammatory cells and destructed or disappeared gland were observed in model group by HE staining, while the inflammation reduced to different degree in CSCC groups. Compared with the model group, CSCC groups expressed significantly higher level of Caspase-1 [model group vs moderate dose CSCC group: (27.02 ± 4.23) ng/L vs (22.73 ± 1.68) ng/L, P ＜ 0.05] and IL-l β [model group vs moderate dose CSCC group: (87.79±11.34) ng/L vs (75.49 ± 8.41) ng/L, P ＜ 0.05]. Conclusion CSCC can reduce the expressions of Caspase-1 and IL-1 β in mice with DSS-induced UC, and alleviate the pathological injury of gut.关键词
五味苦参肠溶胶囊/溃疡性结肠炎/半胱天冬氨酸蛋白酶-1/白介素-1βKey words
composite sophora colon-soluble capsules/ulcerative colitis/Caspase-1/interleukin-1 β分类
医药卫生引用本文复制引用
罗丹,高路,仝战旗,张晨..五味苦参肠溶胶囊对溃疡性结肠炎小鼠半胱天冬氨酸蛋白酶1、白细胞介素-1β蛋白的影响[J].解放军医学院学报,2018,39(2):140-143,149,5.基金项目
国家自然科学基金项目(81673965) （81673965）
Supported by National Natural Science Foundation of China (81673965) （81673965）
