解放军医学院学报2018，Vol.39Issue(3)：234-238,5.DOI:10.3969/j.issn.2095-5227.2018.03.014
负载乙肝核心抗原-黑色素瘤抗原-A3融合蛋白的外周血单个核细胞对人非小细胞肺癌细胞体外杀伤作用的实验
Specific cytotoxic effect of PBMC loaded with HBVc-MAGE-A3 fusion protein on human non-small cell lung cancer cell in vitro
林知 1陈意 1林童俊 2焦顺昌1
作者信息
- 1. 解放军总医院 肿瘤内一科, 北京 100853
- 2. 达尔豪斯大学, 加拿大哈里法克斯 B3K6R8
- 折叠
摘要
Abstract
Objective To evaluate the specific cytotoxic effect of cytotoxic T lymphocyte (CTL) activated by peripheral blood monocyte cell (PBMC) loaded with hepatitis B core antigen (HBVc) -melanoma antigen gene-A3 (MAGE-A3) fusion protein on human non-small cell lung cancer cell with high MAGE-A3 expression in vitro. Methods PBMCs were isolated from peripheral blood of healthy individuals and stimulated with interleukin-2 (IL-2). The PBMCs loaded with HBVc-MAGE-A3 fusion protein were co-cultured with normal PBMCs to induce the specific cytotoxic T lymphocytes. NCI-H358 cell line with high expression of MAGE-A3 antigen was selected as the target cell and HEK-293T cell line with low expression of MAGE-A3 antigen was used as the control cell to carry out the tumor cell killing test. Results Peripheral blood mononuclear cells (PBMC) loaded with HBVc-MAGE-A3 fusion protein antigen could induce specific cytotoxic T lymphocytes (CTLs), and it had significant cytotoxic effect on non-small cell lung cancer cells. The specific killing rate of experimental group was significantly higher than that of the control group (P ＜ 0.05). In NCI-H358 cell group, when the effector target ratio (E: T) was 10: 1, the killing rate of HBVc-MAGE-A3 group was about 60％, while it was about 35％ in both HBVc group and NTC group with significant difference. When E: T = 20: 1, the killing rate of HBVc-MAGE-A3 group was about 90％, significantly higher than 45％ in HBVc group and NTC group. And there was no significant difference between the three groups when using HEK-293T with low expression of MAGE-A3 as target cells. Conclusion Peripheral blood mononuclear cells loaded with HBVc-MAGE-A3 fusion protein antigen can successfully induce specific cytotoxic T lymphocytes (CTLs) and have specific immune killing effect on non-small cell lung cancer cells in vitro.关键词
外周血单个核细胞/黑色素瘤相关抗原-A3/非小细胞肺癌Key words
peripheral blood mononuclear cell/melanoma antigen gene-A3/non-small cell lung cancer分类
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林知,陈意,林童俊,焦顺昌..负载乙肝核心抗原-黑色素瘤抗原-A3融合蛋白的外周血单个核细胞对人非小细胞肺癌细胞体外杀伤作用的实验[J].解放军医学院学报,2018,39(3):234-238,5.
