检验医学与临床2018，Vol.15Issue(12)：1697-1700,4.DOI:10.3969／j.issn.1672-9455.2018.12.001
血清CD146表达在结直肠癌早期诊断中的价值及机制
The value and mechanism of CD146 expression in the early diagnosis of colorectal cancer
摘要
Abstract
Objective To clarify the diagnostic value and mechanism of serum CD146 in early screening of the colorectal cancer.Methods A total of 22 tissue samples of colorectal cancer patients were collected for WB detection of YAP and CD146;CD146 expression was detected by WB after overexpression of YAP in HCT 116 and HT-29 of colon cancer cell lines.A total of 40 cases of healthy persons were taken as comtrol group.A to-tal of 40 cases of adenoma patients were taken as adenoma group and 167 cases of colorectal cancer were taken as colorectal cancer group,serum CD146 concentration was detected by ELISA.Results The expressions of CD146 and its regulatory factor YAP were obviously up-regulated in colorectal carcinoma.The overexpression of YAP in HCT-116 and HT-29 cells can obviously lead to the up-regulation of CD146 expression,and the lev-el of CD146 in the colon cancer group was significantly higher than that in the healthy control group and the adenoma group,and the difference were statistically significant(P＜0.05),and the serum CD146 was signifi-cantly lower than that before the resection of colon cancer.Serum CD146 level was positively correlated with intestinal differentiation,tumor cell infiltration and distant metastasis.Conclusion Serum CD146 can be ex-pected to be a molecular marker for the diagnosis and prognosis of colorectal cancer.The expression of CD146 is closely related to YAP.关键词
结直肠癌/CD146/YAP调控Key words
colorectal cancer/CD146/YAP regulation分类
医药卫生引用本文复制引用
邱芳华,刘道利,龙华婧,杨泽填,刘丽芳..血清CD146表达在结直肠癌早期诊断中的价值及机制[J].检验医学与临床,2018,15(12):1697-1700,4.基金项目
国家自然科学基金资助项目(81503424) （81503424）
广东省自然科学基金资助项目(2014A030313802) （2014A030313802）
广东省广州市产学研协同创新重大专项资助项目(201704020171). （201704020171）
