检验医学与临床2018，Vol.15Issue(12)：1737-1740,4.DOI:10.3969／j.issn.1672-9455.2018.12.012
丙型肝炎患者血清病毒宏基因组学研究
Study on the genome of serum virus in patients with hepatitis C virus
摘要
Abstract
Objective To understand the composition of virus community in serum sample of both healthy people and patients infected with HCV,to compare the difference of composition of serum sample of health group and patient group,and to discover new genotype hepatitis C virus and new virus.Methods All the nu-cleic acids in the plasma samples were extracted and the virus library was amplified by Illumina index primers after reverse transcription.Then the Miseq sequence was sequenced and the virus related sequences were screened by bioinformatics analysis.Results The total number of virus sequences in the HCV01 library was 1 514,including Flaviviridae(48％),Anelloviridae(29％),Retrovirus(7％),Microviridae(2％); the total number of virus sequences in the HCV02 library was 2 820,including Anelloviridae(61％),Flaviviridae(18％),Retrovirus(8％),Microviridae(1％);the total number of virus sequences in the HCV03 library was 3 534,including Anelloviridae(78％),Flaviviridae(4％),Retroviridae(4％),Microviridae(1％); the total number of virus sequences in the HCV04 library was 3 549,including Anelloviridae(90％),Flaviviridae(4％),Retroviridae(2％).Conclusion T here are many kinds of virus in the blood,in the serum of patients in-fected with HCV Anelloviridae,Flaviviridae and Retroviridae are the main viruses,but Anelloviridae and Ret-roviridae are the main viruses in the healthy group.The level of HCV in serum may inhibit the replication of Anelloviridae.The higher the level of the virus,the lower the level of Anelloviridae.关键词
丙型肝炎/病毒宏基因/基因组学/病毒群落Key words
hepatitis C virus/metagenomics/genomics/virus community分类
临床医学引用本文复制引用
李旺,倪萍,周成林..丙型肝炎患者血清病毒宏基因组学研究[J].检验医学与临床,2018,15(12):1737-1740,4.基金项目
江苏省泰州市科技支撑计划(社会发展)资助项目(TS201623) （社会发展）
江苏省泰州市人民医院院级科研基金资助项目(ZL201732). （ZL201732）
