检验医学与临床2018，Vol.15Issue(13)：1892-1895,1898,5.DOI:10.3969/j.issn.1672-9455.2018.13.008
苯那普利和氯沙坦对肾间质纤维化幼鼠组织型转谷氨酰胺酶和转化生长因子-β1的抑制作用
Inhibitory effects of benazepril and losartan on tissue transglutaminase and transforming growth factor β1 in young rats with renal interstitial fibrosis
摘要
Abstract
Objective To investigate the inhibitory effects of benazepril and losartan on tissue transglutam-inase (tTG)and transforming growth factor-β1(TGF-β1) in young rats with renal interstitial fibrosis . Methods Sixty male wistar rats were randomly divided into control group ,model group and intervention group .The model of unilateral ureteral ligation was established in the model group and the intervention group .The intervention group received benazepril and losartan 6 mg/(kg·d) ,1 time/d from the 2nd day after the modeling .And the model group and control group were given the same amount of saline .At the 3rd ,7th , 14th and 28th day after the start of the experiment ,5 mice were sacrificed by HE staining and Masson stai-ning .The non-biotin-labeled PV6001 two-step method was used to detect the expression of tTG and TGF-ex-pression .Results Masson staining showed that the area of interstitial fibrosis was smaller in the control group ,and was significantly increased in the model group ,and was lower in the intervention group than that of the model group (P＜0 .01).The expression of TGF-β1 and tTG was little in the control group ,was high in the model group ,and the expression in intervention group was lower than that of the model group ,the differ-ence was statistically significant (P＜0 .01).The expression of TGF-β1 and tTG in the young rats were in-creased with the time (P＜0 .01).There was a positive correlation between tTG level and Masson staining area (r＝0 .854 ,P＜0 .01).There was a positive correlation between tTG level and TGF-β1 level (r＝0 .813 ,P＜0 .01).Conclusion tTG is positively correlated with TGF-β1,and tTG can induce fibrosis by up-regulating TGF-β1.The benazepril and losartan can play an anti-fibrosis effect by inhibiting TGF-β1 and tTG .关键词
苯那普利/氯沙坦/肾间质纤维化/幼鼠/组织型转谷氨酰胺酶/转化生长因子-β1Key words
benazepril/losartan/renal interstitial fibrosis/young rats/tissue transglutaminase /transforming grow th factor-β1分类
临床医学引用本文复制引用
赵晓华,刘宝环,李冠慧,马宏..苯那普利和氯沙坦对肾间质纤维化幼鼠组织型转谷氨酰胺酶和转化生长因子-β1的抑制作用[J].检验医学与临床,2018,15(13):1892-1895,1898,5.基金项目
河北省沧州市科技支撑计划项目(151302099) （151302099）
