检验医学与临床2018，Vol.15Issue(13)：1903-1905,1909,4.DOI:10.3969/j.issn.1672-9455.2018.13.011
DNALK5抑制乳腺癌增殖的分子机制研究
Research the molecular mechanism of DNALK5 inhibits the proliferation of breast cancer
方娟 1李文鲜 1黄小兰1
作者信息
- 1. 重庆市渝北区人民医院检验科,重庆401120
- 折叠
摘要
Abstract
Objective To investigate whether dominant negative mutation ALK5 can inhibit the prolifera-tion of human breast cancer cell line MDA-MB-231 by inhibiting the TGF-β/SMAD signaling pathway .Meth-ods The expression of ALK5 in breast cancer cells was detected by fluorescence quantitative PCR .The change of proliferation and cycle of breast cancer cells were determined by MTT assay and flow cytometry . The expression of ID1 ,phosphorylation protein and total protein of SMAD 2/3 on TGF-β/SMAD signaling pathway were detected by the western blot while MDA-MB-231 cells were infected with Adenovirus dominant negative ALK5 .Results The results showed that ALK5 mRNA was detected in all kinds of breast cancer cells and the the highest expression breast cancer cell was MDA-MB-231 ;MTT and flow cytometry showed the absorbance of DNALK5 group cell (0 .35 ± 0 .04) was significantly lower than RFP group (0 .58 ± 0 .06) and cell cycle was blocked in G0 stage after Ad DNALK5 infection 3 days .The expression of mRNA and pro-tein of ID1 and CyclinD1 were decreased ,by real time PCR and western blot .further study found that SMAD2/3 phosphorylation protein on TGF-β/SMAD signaling pathway were reduced .Conclusion DNALK5 can inhibit the proliferation of breast cancer cell line MDA-MB-231 through down regulation ID1 and CyclinD1 expression and inhibit the activation of TGF-β/SMAD signaling pathway .关键词
显性负性突变/ALK5/转化生长因子-β/SMAD/乳腺癌/增殖Key words
dominant negative mutation/ALK5/transforming growth factor-β/SMAD/breast cancer/tumor proliferation分类
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方娟,李文鲜,黄小兰..DNALK5抑制乳腺癌增殖的分子机制研究[J].检验医学与临床,2018,15(13):1903-1905,1909,4.
