检验医学与临床2018，Vol.15Issue(13)：1913-1916,4.DOI:10.3969/j.issn.1672-9455.2018.13.014
慢性自发性荨麻疹抗IgE高亲和力受体自身抗体检测方法研究
Study on detection of autoantibodies against IgE high affinity receptor in chronic spontaneous urticaria
徐婷 1易中梅 1罗军梅 1张强 1孟强 1喻荷莲 1王世春 1蒋天伦1
作者信息
- 1. 陆军军医大学第一附属医院输血科,重庆400038
- 折叠
摘要
Abstract
Objective To establish the detection method of anti-human FcεRIα autoantibody ,we obtained the scFv fragment of FcεRIα by phage display .It provided an effective diagnostic method for chronic spontane-ous urticaria in clinical .Methods The target scFv fragments was selected and enriched by phage antibody li-brary .The positive clones were determined by sequencing analysis and expressed in ER2766 .scFv fragment was used to construct the detection of anti-human FcεRIα autoantibodies .Results A total of 12 phage positive clones were obtained and enriched by the phage display .The A20 was converted to ER2766 and the expression product was used to construct the detection of anti-human FcεRIα antibody .The ROC analysis was indicated that the cut off value was 0 .635 ,and its sensitivity and specificity were 96 .7% and 100 .0%,respectively .Con-clusion It provided a power tool for screening the anti-human FcεRIα autoantibodies in clinical research .关键词
慢性自发性荨麻疹/抗FcεRIα自身抗体/酶联免疫吸附试验Key words
chronic spontaneous urticaria/anti-FcεRIα autoantibody/ELISA分类
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徐婷,易中梅,罗军梅,张强,孟强,喻荷莲,王世春,蒋天伦..慢性自发性荨麻疹抗IgE高亲和力受体自身抗体检测方法研究[J].检验医学与临床,2018,15(13):1913-1916,4.
