昆明医科大学学报2018，Vol.39Issue(2)：30-33,4.
云南省食源性沙门菌血清分型及脉冲场凝胶电泳的指纹图谱
Serotyping and PFGE Type of Salmonella Isolates in Yunnan Province
摘要
Abstract
Objective To study the serotypes and molecular characteristics of foodborne Salmonella in Yunnan Province using pulsed field gel electrophoresis (PFGE) and to establish PFGE fingerprint database. Methods This study was carried out on the basis of the Serological typing of 322 strains of foodborne Salmonella which was isolated from Yunnan national foodborne disease surveillance from 2013 to 2016. The clustering analysis was conducted on 148 strains of Salmonella DNA restriction enzyme map by using the software of BioNumerics. Fingerprint database was established through the comparison of clustering analysis correlation on bacterial strain. Results The serotype of 322 strains of Salmonella mainly included A, B, C, D, E, F, G and other 7 groups,among which Salmonella typhimurium was the major type, accounting for 11.4% (37/322) . Cluster analysis was applied using BioNumerics software in 148 strains of Salmonella DNA restriction enzyme map. According to the different number and the different positions, electrophoresis strips were divided into 102 different PFGE patterns (Figure 1) , which was categorized into 39 clusters if 90% of the strips was similar. Conclusion Foodborne salmonella molecular classification is complex. Salmonella typhimurium is the major type. PFGE belt type presents diversity.关键词
沙门菌/血清分型/鼠伤寒沙门菌/脉冲场凝胶电泳/分子分型/食源性致病菌Key words
Salmonella/Serotype/Salmonella typhimurium/Pulsed field gel electrophoresis/Molecular typing/Foodborne pathogen分类
医药卫生引用本文复制引用
赵江,邹颜秋硕,闵向东,蔡同建,韩兴孟,杨祖顺,刘志涛..云南省食源性沙门菌血清分型及脉冲场凝胶电泳的指纹图谱[J].昆明医科大学学报,2018,39(2):30-33,4.基金项目
云南省卫生计生委2012年食品安全保障项目([2012]659号) （[2012]659号）
