昆明医科大学学报2018，Vol.39Issue(6)：35-38,4.
人参皂苷Rg1调控Nrf2在SD大鼠脑缺血再灌注损伤后的抗氧化作用
The Anti-antioxidant Role of Ginsenoside Rg1 Regulating Nrf2 on Focal Cerebral Ischemia Reperfusion SD Rats
摘要
Abstract
Objective To study antioxidant role and mechanism of Rg1 in rats with cerebral ischemia reperfusion injury (IRI).Methods One hundred and twenty healthy male rats were randomly divided into six groups: control group, sham operation group, model group, different concentration (30,60,90 mg/kg) of Rg1 treatment group.MCAO SD rats model was established by suture－occluded method;the Rg1 treatment groups were given Rg1 i.p. in advance, after 24 hours of reperfusion, neurobehavioral scores of all groups were examined by Longa’s standard;The expression of Nrf2 and HO－1 were analyzed by western blot;The content of SOD and MDA was detected by kit.Results The score of model group rats are significantly higher than control group,compared with the model group, the score of different concentration of Rg1 treatment group was decreased (P＜0.05) . The Nrf2 and HO－1 expression in model group was mildly higher than the control or sham group (P＜0.05) . Both of them in every Rg1 treatment group was higher than model group. Compared with control or sham group, SOD content was observably depressed but MDA content was dramatically increased in model group,interestingly,SOD content was enhanced, MDA content was attenuated in different concentration of Rg1 treatment group (P＜0.05). Conclusion Rg1 increases Nrf2 and HO－1 protein expression and SOD content, reduces MDA content,improves neurofunctional performance of rats after MCAO,and alleviates cerebral cerebral IRI.关键词
人生皂苷Rg1/缺血再灌注损伤/血红素加氧酶1/超氧化物歧化酶/丙二醛Key words
Ginsenoside Rg1/Ischemia/Reperfusion injury/Heme oxygenase－1/Superoxide orgotein dis－mutase/Malondialdehyde分类
医药卫生引用本文复制引用
边立功,钟莲梅,艾青龙,陈鑫月,许文凯,闫润淇,邱进,陆地..人参皂苷Rg1调控Nrf2在SD大鼠脑缺血再灌注损伤后的抗氧化作用[J].昆明医科大学学报,2018,39(6):35-38,4.基金项目
国家自然科学基金资助项目(81360200,81460210,81460350,31760292) （81360200,81460210,81460350,31760292）
云南省科技厅－ 昆明医科大学应用基础研究联合专项基金资助项目(2013FB101,2013FB120) （2013FB101,2013FB120）
