昆明医科大学学报2018，Vol.39Issue(6)：101-104,4.
印记基因H19在不同质量精子和胚胎中的甲基化状态
A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos
摘要
Abstract
Objective By comparing the methylation status of imprinted gene H19 in three different quality spermatozoa and the methylation status of the embryos after the combination of spermatozoa, the sperm methylation status in the ART technique was preliminarily explored with the postnatal embryo methylation.Methods In this study, a total of 91 spermatozoa and 91 low－quality embryos were collected from IVF－ET in the First People's Hospital of Yunnan Province. Among them, sperm samples were divided into three groups according to the sperm concentration parameter in the WHO fourth edition,then the relationship between different quality spermatozoa and postnatal embryo methylation were analyzed.Results The abnormality rate of H19 methylation status in group B was significantly higher than that of the sperm group and the embryo group.Conclusions The abnormalities of H19 methylation mainly concentrated in the abnormal parameters of spermatozoa,suggesting that the abnormal state was related to the decrease of sperm quality;Through IVF, ICSI fertilized embryos have the same proportion of the corresponding abnormal state with no significant difference; Abnormal sperm embryos did not show abnormality, which may be related to the self－repair function of the embryo itself.关键词
印记基因H19/精子/胚胎/甲基化/辅助生殖Key words
Imprinting gene H19/Sperm/Embryo/Methylation/ART分类
医药卫生引用本文复制引用
相立峰,王青青,李明颖,曾鹏,李永刚..印记基因H19在不同质量精子和胚胎中的甲基化状态[J].昆明医科大学学报,2018,39(6):101-104,4.基金项目
云南省卫生科技计划基金资助项目(2014NS255) （2014NS255）
