临床误诊误治2018，Vol.31Issue(7)：21-25,5.DOI:10.3969/j.issn.1002-3429.2018.07.006
不同浓度羟考酮对结直肠癌细胞生物学行为的影响
Effects of Different Concentrations of Oxycodone on the Biological Behavior of Colorectal Cancer Cells
摘要
Abstract
Objective To investigate the effect of oxycodone on the biological behavior of colorectal cancer ( CRC) cells. Methods SW620 CRC cells were inoculated and incubated for 24 h, followed by treatment with oxy-codone at 0, 10, 20 and 40 μg/ml for 24 h, and then incubation with CCK-8 kit. The microplate reader was used to measure optical density at a wavelength of 490 nm in different concentration groups, to compare the difference in in-hibition rate of cell proliferation. The effects of oxycodone at different concentrations on apoptosis and migration of SW620 cells were detected by flow cytometry FITC-Annexin V/PI and the scratch test respectively. In addition, the impact of different concentrations of oxycodone on the VEGF expression of SW620 cells was detected by ELISA. Re-sults As compared with the control group ( non-oxycodone treatment group) , oxycodone inhibited the proliferation of apoptosis of SW620 cell in different concentrations of oxycodone treatment groups. There were significant differ-ences in apoptosis of SW620 cells in 20 and 40 μg/ml groups, as compared with that in control group ( t＝12. 64, P＝0. 019; t ＝13. 94, P ＝0. 016 ) . The migration distance of SW620 cells was significantly shorter in 20 and 40 μg/ml groups than in control group(t＝11. 29,P＝0. 036;t＝12. 05,P＝0. 009). The expression levels of VEGF in SW620 cells were significantly lower in 20 and 40 μg/ml groups than in control group(t＝12. 62,P＝0. 031; t＝13. 61,P＝0. 013). Conclusion Oxycodone can suppress the proliferation and migration of CRC cells in a dose-de-pendent manner, inhibit VEGF production and secretion, and induce apoptosis of cells.关键词
羟考酮/结直肠肿瘤/细胞凋亡/细胞增殖/细胞迁移Key words
Oxycodone/Colorectal neoplasms/Apoptosis/Cell proliferation/Cell movement分类
医药卫生引用本文复制引用
贺亚妮,毕鑑红,李清峰,高晓霞,谢元元..不同浓度羟考酮对结直肠癌细胞生物学行为的影响[J].临床误诊误治,2018,31(7):21-25,5.基金项目
陕西省卫生厅科研基金项目(2014JM32541) （2014JM32541）
